View : 48 Download: 6

Zinc-dependent regulation of zinc import and export genes by Zur

Title
Zinc-dependent regulation of zinc import and export genes by Zur
Authors
Choi, Seung-HwanLee, Kang-LokShin, Jung-HoCho, Yoo-BokCha, Sun-ShinRoe, Jung-Hye
Ewha Authors
차선신
SCOPUS Author ID
차선신scopus
Issue Date
2017
Journal Title
NATURE COMMUNICATIONS
ISSN
2041-1723JCR Link
Citation
vol. 8
Publisher
NATURE PUBLISHING GROUP
Indexed
SCI; SCIE; SCOPUS WOS
Abstract
In most bacteria, zinc depletion is sensed by Zur, whereas the surplus is sensed by different regulators to achieve zinc homeostasis. Here we present evidence that zinc-bound Zur not only represses genes for zinc acquisition but also induces the zitB gene encoding a zinc exporter in Streptomyces coelicolor, a model actinobacteria. Zinc-dependent gene regulation by Zur occurs in two phases. At sub-femtomolar zinc concentrations (phase I), dimeric Zur binds to the Zur-box motif immediately upstream of the zitB promoter, resulting in low zitB expression. At the same time, Zur represses genes for zinc uptake. At micromolar zinc concentrations (phase II), oligomeric Zur binding with footprint expansion upward from the Zur box results in high zitB induction. Our findings reveal a mode of zinc-dependent gene activation that uses a single metalloregulator to control genes for both uptake and export over a wide range of zinc concentrations.
DOI
10.1038/ncomms15812
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
Files in This Item:
Zinc-dependent regulation.pdf(2.48 MB)Download
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE