View : 87 Download: 80

Comparison of biochemical properties of the original and newly identified oleate hydratases from Stenotrophomonas maltophilia

Title
Comparison of biochemical properties of the original and newly identified oleate hydratases from Stenotrophomonas maltophilia
Authors
Kang W.-R.Seo M.-J.Shin K.-C.Park J.-B.Oh D.-K.
Ewha Authors
박진병
SCOPUS Author ID
박진병scopus
Issue Date
2017
Journal Title
Applied and Environmental Microbiology
ISSN
0099-2240JCR Link
Citation
vol. 83, no. 9
Keywords
Enzyme characterizationOleate hydrataseStenotrophomonas maltophilia
Publisher
American Society for Microbiology
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Oleate hydratases (OhyAs) catalyze the conversion of unsaturated fatty acids to 10-hydroxy fatty acids, which are used as precursors of important industrial compounds, including lactones and ω-hydroxycarboxylic and α,ω-dicarboxylic acids. The genes encoding OhyA and a putative fatty acid hydratase in Stenotrophomonas maltophilia were identified by genomic analysis. The putative fatty acid hydratase was purified and identified as an oleate hydratase (OhyA2) based on its substrate specificity. The activity of OhyA2 as a holoenzyme was not affected by adding cofactors, whereas the activity of the original oleate hydratase (OhyA1) showed an increase. Thus, all characterized OhyAs were categorized as either OhyA1 or OhyA2 based on the activities of holoenzymes upon adding cofactors, which were determined by the type of the fourth conserved amino acid of flavin adenine dinucleotide (FAD)-binding motif. The hydration activities of S. maltophilia OhyA2 toward unsaturated fatty acids, including oleic acid, palmitoleic acid, linoleic acid, α-linolenic acid, and γ-linolenic acid, were greater than those of OhyA1. Moreover, the specific activity of S. maltophilia OhyA2 toward unsaturated fatty acids, with the exception of γ-linolenic acid, was the highest among all reported OhyAs. © 2017 American Society for Microbiology. All Rights Reserved.
DOI
10.1128/AEM.03351-16
Appears in Collections:
엘텍공과대학 > 식품공학전공 > Journal papers
Files in This Item:
Comparison of biochemical properties.pdf(826.16 kB)Download
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE