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Highly Sensitive Detection of Bisphenol A by NanoAptamer Assay with Truncated Aptamer

Title
Highly Sensitive Detection of Bisphenol A by NanoAptamer Assay with Truncated Aptamer
Authors
Lee, Eun-HeeLim, Hyun JeongLee, Sang-DonSon, Ahjeong
Ewha Authors
이상돈손아정이은희
SCOPUS Author ID
이상돈scopus; 손아정scopus; 이은희scopusscopus
Issue Date
2017
Journal Title
ACS APPLIED MATERIALS & INTERFACES
ISSN
1944-8244JCR Link
Citation
vol. 9, no. 17, pp. 14889 - 14898
Keywords
NanoAptamer assaytruncated aptamerquantum dotsbisphenol Aendocrine-disrupting chemicals
Publisher
AMER CHEMICAL SOC
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
For the sensitive quantification of bisphenol A (BPA), we have developed NanoAptamer assay, which employs aptamer and complementary signaling DNA, a set of quantum dots (QD), and magnetic beads (MBs). Signaling DNAQD(655) was tethered to MBQD(565) via the aptamer. The affinity of the aptamer to BPA resulted in the release of the signaling DNAQD(655) from the complex and hence the corresponding decrease in the QD(655) fluorescence measurement signal. Three new aptamers (23, 58, and 24-mer) were designed via truncation of the reference aptamer (73-mer). The sensitivity and selectivity of each aptamer for BPA detection via NanoAptamer assay were investigated. One of the truncated aptamers (24-mer) has shown a significantly better performance (limit of detection, LOD, 0.17 pg/mL) than the reference 73-mer aptamer (LOD, 570 pg/mL). It has also shown the best selectivity for BPA detection over BPA analogues (i.e., bisphenol B, bisphenol C, and diethylstilbestrol). It corresponded to a normalized fluorescence change of 33.7% at the environmentally relevant concentration of 1 ng/mL (1 ppb) BPA; however, the analogues remained unchanged (2.33.9%).
DOI
10.1021/acsami.7b02377
Appears in Collections:
엘텍공과대학 > 환경공학전공 > Journal papers
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