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Aptamer-coded DNA nanoparticles for targeted doxorubicin delivery using pH-sensitive spacer

Title
Aptamer-coded DNA nanoparticles for targeted doxorubicin delivery using pH-sensitive spacer
Authors
Zhang P.Ye J.Liu E.Sun L.Zhang J.Lee S.-J.Gong J.He H.Yang V.C.
Ewha Authors
이승진
SCOPUS Author ID
이승진scopus
Issue Date
2017
Journal Title
Frontiers of Chemical Science and Engineering
ISSN
2095-0179JCR Link
Citation
pp. 1 - 8
Keywords
aptamerDNA nanoparticlesdoxorubicindrug deliverypH sensitiverolling circle amplification
Publisher
Higher Education Press
Indexed
SCIE; SCOPUS scopus
Abstract
An anticancer drug delivery system consisting of DNA nanoparticles synthesized by rolling circle amplification (RCA) was developed for prostate cancer membrane antigen (PSMA) targeted cancer therapy. The template of RCA was a DNA oligodeoxynucleotide coded with PSMA-targeted aptamer, drug-loading domain, primer binding site and pH-sensitive spacer. Anticancer drug doxorubicin, as the model drug, was loaded into the drug-loading domain (multiple GC-pair sequences) of the DNA nanoparticles by intercalation. Due to the integrated pH-sensitive spacers in the nanoparticles, in an acidic environment, the cumulative release of doxorubicin was far more than the cumulative release of the drug in the normal physiological environment. In cell uptake experiments, treated with doxorubicin loaded DNA nanoparticles, PSMA-positive C4-2 cells could take up more doxorubicin than PSMA-null PC-3 cells. The prepared DNA nanoparticles showed the potential as drug delivery system for PSMA targeting prostate cancer therapy.[Figure not available: see fulltext.] © 2017 Higher Education Press and Springer-Verlag Berlin Heidelberg
DOI
10.1007/s11705-017-1645-z
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약학대학 > 약학과 > Journal papers
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