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(-)-Epigallocatechin-3-gallate stimulates myogenic differentiation through TAZ activation

Title
(-)-Epigallocatechin-3-gallate stimulates myogenic differentiation through TAZ activation
Authors
Kim, A. RumKim, Kyung MinByun, Mi RanHwang, Jun-HaPark, Jung IlOh, Ho TaekJeong, Mi GyeongHwang, Eun SookHong, Jeong-Ho
Ewha Authors
황은숙
SCOPUS Author ID
황은숙scopus
Issue Date
2017
Journal Title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN
0006-291XJCR Link1090-2104JCR Link
Citation
vol. 486, pp. 378 - 384
Keywords
CatechinsMyogenesisSatellite cellsTAZ
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Muscle loss is a typical process of aging. Green tea consumption is known to slow down the progress of aging. Their underlying mechanisms, however, remain largely unknown. In this study, we investigated the effect of (-)-epigallocatechin-3-gallate (EGCG), a polyphenolic compound of green tea, on myogenic differentiation and found that EGCG significantly increases myogenic differentiation. After EGCG treatment, the expression of myogenic marker genes, such as myosin heavy chain, are increased through activation of TAZ, a transcriptional coactivator with a PDZ-binding motif. TAZ-knockdown does not stimulate EGCG-induced myogenic differentiation. EGCG facilitates the interaction between TAZ and MyoD, which stimulates MyoD-mediated gene transcription. EGCG induces nuclear localization of TAZ through the dephosphorylation of TAZ at its Ser89 residue, which relieves 14-3-3 binding in the cytosol. Interestingly, inactivation of Lats kinase is observed after EGCG treatment, which is responsible for the production of dephosphorylated TAZ. Together, these results suggest that EGCG induces myogenic differentiation through TAZ, suggesting that TAZ plays an important role in EGCG induced muscle regeneration. (C) 2017 Elsevier Inc. All rights reserved.
DOI
10.1016/j.bbrc.2017.03.049
Appears in Collections:
약학대학 > 약학과 > Journal papers
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