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Genetic and Structural Characterization of a Thermo-Tolerant, Cold-Active, and Acidic Endo-beta-1,4-glucanase from Antarctic Springtail, Cryptopygus antarcticus
- Title
- Genetic and Structural Characterization of a Thermo-Tolerant, Cold-Active, and Acidic Endo-beta-1,4-glucanase from Antarctic Springtail, Cryptopygus antarcticus
- Authors
- Song, Jung Min; Hong, Seung Kon; An, Young Jun; Kang, Mee Hye; Hong, Kwon Ho; Lee, Youn-Ho; Cha, Sun-Shin
- Ewha Authors
- 차선신
- SCOPUS Author ID
- 차선신
- Issue Date
- 2017
- Journal Title
- JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
- ISSN
- 0021-8561
1520-5118
- Citation
- JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY vol. 65, no. 8, pp. 1630 - 1640
- Keywords
- cold-active cellulase; Cryptopygus antarcticus; endo-beta-1,4-glucanase; horizontal gene transfer; biochemical and structural features; biofuel production from seaweeds
- Publisher
- AMER CHEMICAL SOC
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- The CaCel gene from Antarctic springtail Cryptopygus antarcticus codes for a cellulase belonging to the glycosyl hydrolase family 45 (GHF45). Phylogenetic, biochemical, and structural analyses revealed that the CaCel gene product (CaCel) is closely related to fungal GHF45 endo-,beta-1,4-glucanases. The organization of five introns within the open reading frame of the CaCel gene indicates its endogenous origin in the genome of the species, which suggests the horizontal transfer of the gene from fungi to the springtail. CaCel exhibited optimal activity at pH 3.5, retained 80% of its activity at 0-10 degrees C, and maintained a half-life of 4 h at 70 degrees C. Based on the structural comparison between CaCel and a fungal homologue, we deduced the structural basis for the unusual characteristics of CaCel. Under acidic conditions at 50 degrees C, CaCel was effective to digest the green algae (Viva pertusa), suggesting that it could be exploited for biofuel production from seaweeds.
- DOI
- 10.1021/acsjafc.6b05037
- Appears in Collections:
- 자연과학대학 > 화학·나노과학전공 > Journal papers
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