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Genetic and Structural Characterization of a Thermo-Tolerant, Cold-Active, and Acidic Endo-beta-1,4-glucanase from Antarctic Springtail, Cryptopygus antarcticus

Title
Genetic and Structural Characterization of a Thermo-Tolerant, Cold-Active, and Acidic Endo-beta-1,4-glucanase from Antarctic Springtail, Cryptopygus antarcticus
Authors
Song, Jung MinHong, Seung KonAn, Young JunKang, Mee HyeHong, Kwon HoLee, Youn-HoCha, Sun-Shin
Ewha Authors
차선신
SCOPUS Author ID
차선신scopus
Issue Date
2017
Journal Title
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN
0021-8561JCR Link1520-5118JCR Link
Citation
vol. 65, no. 8, pp. 1630 - 1640
Keywords
cold-active cellulaseCryptopygus antarcticusendo-beta-1,4-glucanasehorizontal gene transferbiochemical and structural featuresbiofuel production from seaweeds
Publisher
AMER CHEMICAL SOC
Indexed
SCI; SCIE; SCOPUS WOS
Abstract
The CaCel gene from Antarctic springtail Cryptopygus antarcticus codes for a cellulase belonging to the glycosyl hydrolase family 45 (GHF45). Phylogenetic, biochemical, and structural analyses revealed that the CaCel gene product (CaCel) is closely related to fungal GHF45 endo-,beta-1,4-glucanases. The organization of five introns within the open reading frame of the CaCel gene indicates its endogenous origin in the genome of the species, which suggests the horizontal transfer of the gene from fungi to the springtail. CaCel exhibited optimal activity at pH 3.5, retained 80% of its activity at 0-10 degrees C, and maintained a half-life of 4 h at 70 degrees C. Based on the structural comparison between CaCel and a fungal homologue, we deduced the structural basis for the unusual characteristics of CaCel. Under acidic conditions at 50 degrees C, CaCel was effective to digest the green algae (Viva pertusa), suggesting that it could be exploited for biofuel production from seaweeds.
DOI
10.1021/acsjafc.6b05037
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자연과학대학 > 화학·나노과학전공 > Journal papers
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