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Sublingual immunization with Japanese encephalitis virus vaccine effectively induces immunity through both cellular and humoral immune responses in mice

Title
Sublingual immunization with Japanese encephalitis virus vaccine effectively induces immunity through both cellular and humoral immune responses in mice
Authors
Lee, Eun-YoungKim, Joo-YoungLee, Deuk-KiYoon, Il-SubKo, Hae LiChung, Ji-WooChang, JunNam, Jae-Hwan
Ewha Authors
장준김주영
SCOPUS Author ID
장준scopus; 김주영scopus
Issue Date
2016
Journal Title
MICROBIOLOGY AND IMMUNOLOGY
ISSN
0385-5600JCR Link1348-0421JCR Link
Citation
vol. 60, no. 12, pp. 846 - 853
Keywords
intramuscularJapanese encephalitis virusSA14-14-2sublingual
Publisher
WILEY-BLACKWELL
Indexed
SCI; SCIE; SCOPUS WOS
Abstract
The Japanese encephalitis virus (JEV) is the leading cause of viral encephalitis. Although there are four classes of vaccines against JEV, all of them are administered by s.c or i.m injection. Here, the effectiveness of sublingual (s.l.) administration of a JEV live-attenuated vaccine or recombinant modified vaccinia virus Ankara (MVA) vaccine, including JEV prM/E, was investigated. The mice were immunized three times i.m. or s.c. One week after the final immunization by both s.l. and i.m. routes, the titers of IgG1 induced by the recombinant MVA vaccine were higher than those induced by the live-attenuated vaccine, whereas the titers of IgG2a induced by the live-attenuated vaccine were higher than those induced by the recombinant MVA vaccine. However, both vaccines induced neutralizing antibodies when given by either s.l. or i.m. routes, indicating that both vaccines induce appropriate Th1 and Th2 cell responses through the s.l. and i.m. routes. Moreover, both vaccines protected against induction of proinflammatory cytokines and focal spleen white pulp hyperplasia after viral challenge. Virus-specific IFN-gamma(+) CD4(+) and CD8(+) T cells appeared to increase in mice immunized via both s.l. and i.m. routes. Interestingly, virus-specific IL-17(+) CD4(+) T cells increased significantly only in the mice immunized via the s.l. route; however, the increased IL-17 did not affect pathogenicity after viral challenge. These results suggest that s.l. immunization may be as useful as i.m. injection for induction of protective immune responses against JEV by both live-attenuated and recombinant MVA vaccines.
DOI
10.1111/1348-0421.12458
Appears in Collections:
약학대학 > 약학과 > Journal papers
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