Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 신동해 | - |
dc.contributor.author | 김수원 | - |
dc.date.accessioned | 2017-03-01T01:03:01Z | - |
dc.date.available | 2017-03-01T01:03:01Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 2053-230X | - |
dc.identifier.other | OAK-20197 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/234649 | - |
dc.description.abstract | The Gram-negative bacterium Burkholderia pseudomallei is the causative agent of melioidosis. D-glycero-beta-D-manno-Heptose-1-phosphate adenylyltransferase (HldC) is the fourth enzyme of the ADP-L-glycero-beta-D-manno-heptose biosynthesis pathway, which produces an essential carbohydrate comprising the inner core of lipopolysaccharide. Therefore, HldC is a potential target of antibiotics against melioidosis. In this study, HldC from B. pseudomallei has been cloned, expressed, purified and crystallized. Synchrotron X-ray data from a selenomethionine-substituted HldC crystal were also collected to 2.8 angstrom resolution. The crystal belonged to the primitive triclinic space group P1, with unit-cell parameters a = 74.0, b = 74.0, c = 74.9 angstrom, alpha = 108.4, beta = 108.4, gamma = 108.0 degrees. Eight protomers are present in the unit cell and three out of five selenomethionines were found in each protomer using the PHENIX software suite. A full structural determination is in progress to elucidate the structurefunction relationship of the protein. | - |
dc.language | English | - |
dc.publisher | INT UNION CRYSTALLOGRAPHY | - |
dc.subject | Burkholderia pseudomallei | - |
dc.subject | heptose biosynthesis pathway | - |
dc.subject | melioidosis | - |
dc.subject | D-glycero-beta-D-manno-heptose-1-phosphate adenylyltransferase | - |
dc.subject | HldC | - |
dc.title | Expression and crystallographic studies of D-glycero-beta-D-manno-heptose-1-phosphate adenylyltransferase from Burkholderia pseudomallei | - |
dc.type | Article | - |
dc.relation.volume | 73 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 90 | - |
dc.relation.lastpage | 94 | - |
dc.relation.journaltitle | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | - |
dc.identifier.doi | 10.1107/S2053230X16020537 | - |
dc.identifier.wosid | WOS:000396915200005 | - |
dc.identifier.scopusid | 2-s2.0-85011982944 | - |
dc.author.google | Park, Jimin | - |
dc.author.google | Kim, Hyojin | - |
dc.author.google | Kim, Suwon | - |
dc.author.google | Lee, Daeun | - |
dc.author.google | Shin, Dong Hae | - |
dc.contributor.scopusid | 신동해(57217374185) | - |
dc.contributor.scopusid | 김수원(57193241841) | - |
dc.date.modifydate | 20230411112717 | - |