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Non-invasive stem cell tracking in hindlimb ischemia animal model using bio-orthogonal copper-free click chemistry

Title
Non-invasive stem cell tracking in hindlimb ischemia animal model using bio-orthogonal copper-free click chemistry
Authors
Lee S.Y.Lee S.Lee J.Yhee J.Y.Yoon H.I.Park S.-J.Koo H.Moon S.-H.Lee H.Cho Y.W.Kang S.W.Lee S.-Y.Kim K.
Ewha Authors
이혁진
SCOPUS Author ID
이혁진scopus
Issue Date
2016
Journal Title
Biochemical and Biophysical Research Communications
ISSN
0006-291XJCR Link
Citation
vol. 479, no. 4, pp. 779 - 786
Keywords
Bio-orthogonal copper-free click chemistryCell labeling and trackingHindlimb ischemiaMesenchymal stem cellMetabolic glycoengineering
Publisher
Elsevier B.V.
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Labeling of stem cells aims to distinguish transplanted cells from host cells, understand in vivo fate of transplanted cells, particularly important in stem cell therapy. Adipose-derived mesenchymal stem cells (ASCs) are considered as an emerging therapeutic option for tissue regeneration, but much remains to be understood regarding the in vivo evidence. In this study, a simple and efficient cell labeling method for labeling and tracking of stem cells was developed based on bio-orthogonal copper-free click chemistry, and it was applied in a mouse hindlimb ischemia model. The human ASCs were treated with tetra-acetylated N-azidoacetyl-D-mannosamine (Ac4ManNAz) to generate glycoprotein with unnatural azide groups on the cell surface, and the generated azide groups were fluorescently labeled by specific binding of dibenzylcyclooctyne-conjugated Cy5 (DBCO-Cy5). The safe and long-term labeling of the hASCs by this method was first investigated in vitro. Then the DBCO-Cy5-hASCs were transplanted into the hindlimb ischemia mice model, and we could monitor and track in vivo fate of the cells using optical imaging system. We could clearly observe the migration potent of the hASCs toward the ischemic lesion. This approach to design and tailor new method for labeling of stem cells may be useful to provide better understanding on the therapeutic effects of transplanted stem cells into the target diseases. © 2016 Elsevier Inc.
DOI
10.1016/j.bbrc.2016.09.132
Appears in Collections:
약학대학 > 약학과 > Journal papers
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