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Influence of culture conditions on Escherichia coli O157:H7 biofilm formation by atomic force microscopy

Title
Influence of culture conditions on Escherichia coli O157:H7 biofilm formation by atomic force microscopy
Authors
Oh Y.J.Jo W.Yang Y.Park S.
Ewha Authors
조윌렴박성수
SCOPUS Author ID
조윌렴scopus
Issue Date
2007
Journal Title
Ultramicroscopy
ISSN
0304-3991JCR Link
Citation
vol. 107, no. 41558, pp. 869 - 874
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Biofilms are complex microbial communities that are resistant against attacks by bacteriophages and removal by drugs and chemicals. In this study, biofilms of Escherichia coli O157:H7, a bacterial pathogen, were investigated using atomic force microscopy (AFM) in terms of the dynamic transition of morphology and surface properties of bacterial cells over the development of biofilms. The physical and topographical properties of biofilms are different, depending on nutrient availability. Compared to biofilms formed in a high nutrient medium, biofilms form faster and a higher number of bacterial cells were recovered on glass surface in a low nutrient medium. We demonstrate that AFM can obtain high-resolution images and the elastic information about biofilms. As E. coli biofilm becomes mature, the magnitude of the force between a tip and the surface of the biofilm gets stronger, suggesting that extracellular polymeric substances (EPSs), sticky components of biofilms, accumulate over the surface of cells upon the initial attachment of bacterial cells to surfaces. © 2007 Elsevier B.V. All rights reserved.
DOI
10.1016/j.ultramic.2007.01.021
Appears in Collections:
자연과학대학 > 물리학전공 > Journal papers
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