Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박진병 | * |
dc.date.accessioned | 2017-02-15T08:02:33Z | - |
dc.date.available | 2017-02-15T08:02:33Z | - |
dc.date.issued | 2007 | * |
dc.identifier.issn | 0175-7598 | * |
dc.identifier.other | OAK-4157 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/234409 | - |
dc.description.abstract | Whole-cell conversion of cyclohexanone to ε-caprolactone was attempted by recombinant Escherichia coli BL21(DE3) expressing cyclohexanone monooxygenase (CHMO) of Acinetobacter calcoaceticus NCIMB 9871. High concentrations of cyclohexanone and ε-caprolactone reduced CHMO-mediated bioconversion of cyclohexanone to ε-caprolactone in the resting recombinant E. coli cells. Metabolically active cells were employed by adopting a fed-batch culture to improve the production of ε-caprolactone from cyclohexanone. A glucose-limited fed-batch Baeyer-Villiger oxidation where a cyclohexanone level was maintained less than 6 g/l resulted in a maximum ε-caprolactone concentration of 11.0 g/l. The maximum ε-caprolactone concentration was improved further to 15.3 g/l by coexpression of glucose-6-phosphate dehydrogenase, an NADPH-generating enzyme encoded by the zwf gene which corresponded to a 39% enhancement in ε-caprolactone concentration compared with the control experiment performed under the same conditions. © 2007 Springer-Verlag. | * |
dc.language | English | * |
dc.title | Enhanced production of ε-caprolactone by overexpression of NADPH-regenerating glucose 6-phosphate dehydrogenase in recombinant Escherichia coli harboring cyclohexanone monooxygenase gene | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 76 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 329 | * |
dc.relation.lastpage | 338 | * |
dc.relation.journaltitle | Applied Microbiology and Biotechnology | * |
dc.identifier.doi | 10.1007/s00253-007-1016-7 | * |
dc.identifier.wosid | WOS:000248299800009 | * |
dc.identifier.scopusid | 2-s2.0-34547114478 | * |
dc.author.google | Lee W.-H. | * |
dc.author.google | Park J.-B. | * |
dc.author.google | Park K. | * |
dc.author.google | Kim M.-D. | * |
dc.author.google | Seo J.-H. | * |
dc.contributor.scopusid | 박진병(15036390700) | * |
dc.date.modifydate | 20240322114808 | * |