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Clustering of peptidoglycan recognition protein-SA is required for sensing lysine-type peptidoglycan in insects

Title
Clustering of peptidoglycan recognition protein-SA is required for sensing lysine-type peptidoglycan in insects
Authors
Park J.-W.Kim C.-H.Kim J.-H.Je B.-R.Roh K.-B.Kim S.-J.Lee H.-H.Ryu J.-H.Lim J.-H.Oh B.-H.Lee W.-J.Ha N.-C.Lee B.-L.
Ewha Authors
이원재
Issue Date
2007
Journal Title
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424JCR Link
Citation
vol. 104, no. 16, pp. 6602 - 6607
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Recognition of lysine-type peptidoglycan by peptidoglycan recognition protein (PGRP)-SA provokes the activation of the Toll and prophenoloxidase pathways. Here we reveal that a soluble fragment of lysine-type peptidoglycan, a long glycan chain with short stem peptides, is a potent activator of the Drosophila Toll pathway and the prophenoloxidase activation cascade in the beetle Tenebrio molitor. Using this peptidoglycan fragment, we present biochemical evidence that clustering of PGRP-SA molecules on the peptidoglycan is required for the activation of the prophenoloxidase cascade. We subsequently highlight that the lysozyme-mediated partial digestion of highly cross-linked lysine-type peptidoglycan dramatically increases the binding of PGRP-SA, presumably by inducing clustering of PGRP-SA, which then recruits the Gram-negative bacteria-binding protein 1 homologue and a modular serine protease containing low-density lipoprotein and complement control protein domains. The crucial role of lysozyme in the prophenoloxidase activation cascade is further confirmed in vivo by using a lysozyme inhibitor. Taken together, we propose a model whereby lysozyme presents a processed form of lysine-type peptidoglycan for clustering of PGRP-SA that recruits Gram-negative bacteria-binding protein 1 and the modular serine protease, which leads to the activation of both the Toll and prophenoloxidase pathways. © 2007 by The National Academy of Sciences of the USA.
DOI
10.1073/pnas.0610924104
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일반대학원 > 바이오융합과학과 > Journal papers
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