Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 이원재 | - |
dc.date.accessioned | 2017-02-15T08:02:11Z | - |
dc.date.available | 2017-02-15T08:02:11Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.other | OAK-3955 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/234304 | - |
dc.description.abstract | Recognition of lysine-type peptidoglycan by peptidoglycan recognition protein (PGRP)-SA provokes the activation of the Toll and prophenoloxidase pathways. Here we reveal that a soluble fragment of lysine-type peptidoglycan, a long glycan chain with short stem peptides, is a potent activator of the Drosophila Toll pathway and the prophenoloxidase activation cascade in the beetle Tenebrio molitor. Using this peptidoglycan fragment, we present biochemical evidence that clustering of PGRP-SA molecules on the peptidoglycan is required for the activation of the prophenoloxidase cascade. We subsequently highlight that the lysozyme-mediated partial digestion of highly cross-linked lysine-type peptidoglycan dramatically increases the binding of PGRP-SA, presumably by inducing clustering of PGRP-SA, which then recruits the Gram-negative bacteria-binding protein 1 homologue and a modular serine protease containing low-density lipoprotein and complement control protein domains. The crucial role of lysozyme in the prophenoloxidase activation cascade is further confirmed in vivo by using a lysozyme inhibitor. Taken together, we propose a model whereby lysozyme presents a processed form of lysine-type peptidoglycan for clustering of PGRP-SA that recruits Gram-negative bacteria-binding protein 1 and the modular serine protease, which leads to the activation of both the Toll and prophenoloxidase pathways. © 2007 by The National Academy of Sciences of the USA. | - |
dc.language | English | - |
dc.title | Clustering of peptidoglycan recognition protein-SA is required for sensing lysine-type peptidoglycan in insects | - |
dc.type | Article | - |
dc.relation.issue | 16 | - |
dc.relation.volume | 104 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 6602 | - |
dc.relation.lastpage | 6607 | - |
dc.relation.journaltitle | Proceedings of the National Academy of Sciences of the United States of America | - |
dc.identifier.doi | 10.1073/pnas.0610924104 | - |
dc.identifier.wosid | WOS:000245869200022 | - |
dc.identifier.scopusid | 2-s2.0-34249851525 | - |
dc.author.google | Park J.-W. | - |
dc.author.google | Kim C.-H. | - |
dc.author.google | Kim J.-H. | - |
dc.author.google | Je B.-R. | - |
dc.author.google | Roh K.-B. | - |
dc.author.google | Kim S.-J. | - |
dc.author.google | Lee H.-H. | - |
dc.author.google | Ryu J.-H. | - |
dc.author.google | Lim J.-H. | - |
dc.author.google | Oh B.-H. | - |
dc.author.google | Lee W.-J. | - |
dc.author.google | Ha N.-C. | - |
dc.author.google | Lee B.-L. | - |
dc.contributor.scopusid | 이원재(57171092600) | - |
dc.date.modifydate | 20211210153636 | - |