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Production of aminoglycosides in non-aminoglycoside producing Streptomyces lividans TK24

Title: Production of aminoglycosides in non-aminoglycoside producing Streptomyces lividans TK24

Authors: Subba B.; Kurumbang N.P.; Jung Y.S.; Yoon Y.J.; Lee H.C.; Liou K.; Sohng J.K.

Citation: Bioorganic and Medicinal Chemistry Letters vol. 17, no. 7, pp. 1892 - 1896

Abstract: The pRBM4 cosmid, which harbors a putative cluster of genes spanning a 31.8-kb chromosomal region of the ribostamycin producer Streptomyces ribosidificus ATCC 21294, was heterologously expressed in Streptomyces lividans TK24. ESI-MS/MS, HPLC, and LC-ESI MS analyses showed that the transformation gave rise to ribostamycin production in various culture broths. This is the first report of heterologous aminoglycoside production. © 2007 Elsevier Ltd. All rights reserved.