Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 윤여준 | - |
dc.date.accessioned | 2017-02-15T08:02:11Z | - |
dc.date.available | 2017-02-15T08:02:11Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1364-5072 | - |
dc.identifier.other | OAK-3947 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/234301 | - |
dc.description.abstract | Aims: Cytochrome P450 monooxygenases (CYPs) are useful catalysts for oxidation reactions. Self-sufficient CYPs harbour a reductive domain covalently connected to a P450 domain and are known for their robust catalytic activity with great potential as biocatalysts. In an effort to expand genetic sources of self-sufficient CYPs, we devised a sequence-based screening system to identify them in a soil metagenome. Methods and Results: We constructed a soil metagenome library and performed sequence-based screening for self-sufficient CYP genes. A new CYP gene, syk181, was identified from the metagenome library. Phylogenetic analysis revealed that SYK181 formed a distinct phylogenic line with 46% amino-acid-sequence identity to CYP102A1 which has been extensively studied as a fatty acid hydroxylase. The heterologously expressed SYK181 showed significant hydroxylase activity towards naphthalene and phenanthrene as well as towards fatty acids. Conclusions: Sequence-based screening of metagenome libraries is expected to be a useful approach for searching self-sufficient CYP genes. The translated product of syk181 shows self-sufficient hydroxylase activity towards fatty acids and aromatic compounds. Significance and Impact of the Study: SYK181 is the first self-sufficient CYP obtained directly from a metagenome library. The genetic and biochemical information on SYK181 are expected to be helpful for engineering self-sufficient CYPs with broader catalytic activities towards various substrates, which would be useful for bioconversion of natural products and biodegradation of organic chemicals. © 2007 The Authors. | - |
dc.language | English | - |
dc.title | Sequence-based screening for self-sufficient P450 monooxygenase from a metagenome library | - |
dc.type | Article | - |
dc.relation.issue | 5 | - |
dc.relation.volume | 102 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 1392 | - |
dc.relation.lastpage | 1400 | - |
dc.relation.journaltitle | Journal of Applied Microbiology | - |
dc.identifier.doi | 10.1111/j.1365-2672.2006.03169.x | - |
dc.identifier.wosid | WOS:000245812700023 | - |
dc.identifier.scopusid | 2-s2.0-34247365934 | - |
dc.author.google | Kim B.S. | - |
dc.author.google | Kim S.Y. | - |
dc.author.google | Park J. | - |
dc.author.google | Park W. | - |
dc.author.google | Hwang K.Y. | - |
dc.author.google | Yoon Y.J. | - |
dc.author.google | Oh W.K. | - |
dc.author.google | Kim B.Y. | - |
dc.author.google | Ahn J.S. | - |
dc.contributor.scopusid | 윤여준(7402126465) | - |
dc.date.modifydate | 20210708161345 | - |