Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 정준모 | * |
dc.date.accessioned | 2017-02-15T08:02:04Z | - |
dc.date.available | 2017-02-15T08:02:04Z | - |
dc.date.issued | 2007 | * |
dc.identifier.issn | 0006-8993 | * |
dc.identifier.other | OAK-3875 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/234270 | - |
dc.description.abstract | We previously demonstrated that ginsenoside Rg3 (Rg3), one of the active ingredients in Panax ginseng, attenuates NMDA receptor-mediated currents and NMDA-induced neurotoxicity (Kim, S., Kim, T., Ahn, K., Park, W.K., Nah, S.Y., Rhim, H., 2004. Ginsenoside Rg3 antagonizes NMDA receptors through a glycine modulatory site in rat cultured hippocampal neurons. Biochem. Biophys. Res. Commun. 323, 416-424). Accumulating evidence suggests that homocysteine (HC), a metabolite of methionine, exerts its excitotoxicity through NMDA receptor activation. In the present study, we examined the neuroprotective effects of Rg3 on HC-induced hippocampal excitotoxicity in vitro and in vivo. Our in vitro studies using rat cultured hippocampal neurons revealed that Rg3 treatment significantly and dose-dependently inhibited HC-induced hippocampal cell death, with an EC50 value of 28.7 ± 7.5 μM. Rg3 treatment not only significantly reduced HC-induced DNA damage, but also dose-dependently attenuated HC-induced caspase-3 activity in vitro. Our in vivo studies revealed that intracerebroventricular (i.c.v.) pre-administration of Rg3 significantly and dose-dependently reduced i.c.v. HC-induced hippocampal damage in rats. To examine the mechanisms underlying the in vitro and in vivo neuroprotective effects of Rg3 against HC-induced hippocampal excitotoxicity, we examined the effect of Rg3 on HC-induced intracellular Ca2+ elevations in cultured hippocampal cells and found that Rg3 treatment dose-dependently inhibited HC-induced intracellular Ca2+ elevation, with an IC50 value of 41.5 ± 17.5 μM. In addition, Rg3 treatment dose-dependently inhibited HC-induced currents in Xenopus oocytes expressing the NMDA receptor, with an IC50 of 47.3 ± 14.2 μM. These results collectively indicate that Rg3-induced neuroprotection against HC in rat hippocampus might be achieved via inhibition of HC-mediated NMDA receptor activation. © 2006 Elsevier B.V. All rights reserved. | * |
dc.language | English | * |
dc.title | Neuroprotective effects of ginsenoside Rg3 against homocysteine-induced excitotoxicity in rat hippocampus | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 1136 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 190 | * |
dc.relation.lastpage | 199 | * |
dc.relation.journaltitle | Brain Research | * |
dc.identifier.doi | 10.1016/j.brainres.2006.12.047 | * |
dc.identifier.wosid | WOS:000244882500018 | * |
dc.identifier.scopusid | 2-s2.0-33846882015 | * |
dc.author.google | Kim J.-H. | * |
dc.author.google | Cho S.Y. | * |
dc.author.google | Lee J.-H. | * |
dc.author.google | Jeong S.M. | * |
dc.author.google | Yoon I.-S. | * |
dc.author.google | Lee B.-H. | * |
dc.author.google | Pyo M.K. | * |
dc.author.google | Lee S.-M. | * |
dc.author.google | Chung J.-M. | * |
dc.author.google | Kim S. | * |
dc.author.google | Rhim H. | * |
dc.author.google | Oh J.-W. | * |
dc.author.google | Nah S.-Y. | * |
dc.contributor.scopusid | 정준모(9233608000) | * |
dc.date.modifydate | 20240415121825 | * |