View : 126 Download: 0
Receptor activator of nuclear factor (NF)-κB ligand (RANKL) increases vascular permeability: Impaired permeability and angiogenesis in eNOS-deficient mice
- Receptor activator of nuclear factor (NF)-κB ligand (RANKL) increases vascular permeability: Impaired permeability and angiogenesis in eNOS-deficient mice
- Min J.-K.; Cho Y.-L.; Choi J.-H.; Kim Y.; Jeong H.K.; Young S.Y.; Rho J.; Mochizuki N.; Kim Y.-M.; Goo T.O.; Kwon Y.-G.
- Ewha Authors
- SCOPUS Author ID
- Issue Date
- Journal Title
- vol. 109, no. 4, pp. 1495 - 1502
- SCI; SCIE; SCOPUS
- Receptor activator of nuclear factor (NF)-κB ligand (RANKL) is emerging as an important regulator of vascular pathophysiology. Here, we demonstrate a novel role of RANKL as a vascular permeability factor and a critical role of endothelial nitric oxide synthase (eNOS) in RANKL-induced endothelial function. RANKL increased the vascular permeability and leukocyte infiltration in vivo and caused the breakdown of the blood-retinal barrier in wild-type mice but not in eNOS-deficient mice. In vitro, it increased endothelial permeability and reduced VE-cadherin-facilitated endothelial cell-cell junctions in a NO-dependent manner. RANKL also led to the activation of Akt and eNOS and to NO production in endothelial cells (ECs). These effects were suppressed by the inhibition of TRAF6, phosphoinositide 3′-kinase (PI3K), Akt, or NOS by genetic or pharmacologic means. Inhibition of the TRAF6-mediated NO pathway reduced EC migration and capillary-like tube formation in response to RANKL. Moreover, the effects of RANKL on ECs sprouting from the aorta, and neovessel formation in both the mouse Matrigel plug assay and corneal micropocket assay, were impaired in eNOS-deficient mice. These results demonstrate that RANKL promotes vascular permeability and angiogenesis by stimulating eNOS by a TRAF6-PI3K-Akt-dependent mechanism. These properties may be relevant to the pathogenesis of angiogenesis-dependent and inflammatory vascular diseases. © 2007 by The American Society of Hematology.
- Appears in Collections:
- 자연과학대학 > 생명과학전공 > Journal papers
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.