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DRE-CALUX bioassay in comparison with HRGC/MS for measurement of toxic equivalence in environmental samples
- DRE-CALUX bioassay in comparison with HRGC/MS for measurement of toxic equivalence in environmental samples
- Joung K.E.; Chung Y.H.; Sheen Y.Y.
- Ewha Authors
- SCOPUS Author ID
- Issue Date
- Journal Title
- Science of the Total Environment
- Science of the Total Environment vol. 372, no. 41308, pp. 657 - 667
- SCI; SCIE; SCOPUS
- Document Type
- CALUX, Chemically Activated LUciferase gene eXpression bioassay, has proven valuable for screening for and assessing toxic equivalents of dioxin-like compounds, because it detects all AhR (arylhydrocarbon receptor) ligands in a variety of sample matrices. In this study, we tried to validate DRE (dioxin-response elements)-CALUX bioassay, which has been developed by cloning mouse cyp1a1 gene in front of luciferase reporter gene. We compared DRE-CALUX bioassay with high resolution gas chromatography/mass spectrometry (HRGC/MS) for assessing environmental samples from Korea. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) dose response study showed high correlation (r2 = 0.89) between DRE-CALUX bioassay and EROD (ethoxyresorufin O-deethylase) bioassay, a commonly used bioassay method. The mean TEQ value of water samples was 0.57 pg-TEQHRGC/MS/L and 4.97 pg-TEQCALUX/L. For soil samples, HRGC/MS-TEQ values ranged from 0 to 47.18 pg-TEQ/g (dry) and correlated well (r2 = 0.98) with values obtained by CALUX-TEQ which ranged from 0.92 to 649.97 pg-TEQ/g (dry). The difference between the absolute TEQ values might be due to the presence of dioxin-like compounds without WHO-TEQ values rather than the difference between CALUX-REP and WHO-TEQ. Based on this study, we suggest that DRE-CALUX bioassay can serve as an alternative bioassay method for high-throughput analysis of large number of environmental samples. © 2006 Elsevier B.V. All rights reserved.
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