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DRE-CALUX bioassay in comparison with HRGC/MS for measurement of toxic equivalence in environmental samples

Title
DRE-CALUX bioassay in comparison with HRGC/MS for measurement of toxic equivalence in environmental samples
Authors
Joung K.E.Chung Y.H.Sheen Y.Y.
Ewha Authors
신윤용
SCOPUS Author ID
신윤용scopus
Issue Date
2007
Journal Title
Science of the Total Environment
ISSN
0048-9697JCR Link
Citation
vol. 372, no. 41308, pp. 657 - 667
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
CALUX, Chemically Activated LUciferase gene eXpression bioassay, has proven valuable for screening for and assessing toxic equivalents of dioxin-like compounds, because it detects all AhR (arylhydrocarbon receptor) ligands in a variety of sample matrices. In this study, we tried to validate DRE (dioxin-response elements)-CALUX bioassay, which has been developed by cloning mouse cyp1a1 gene in front of luciferase reporter gene. We compared DRE-CALUX bioassay with high resolution gas chromatography/mass spectrometry (HRGC/MS) for assessing environmental samples from Korea. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) dose response study showed high correlation (r2 = 0.89) between DRE-CALUX bioassay and EROD (ethoxyresorufin O-deethylase) bioassay, a commonly used bioassay method. The mean TEQ value of water samples was 0.57 pg-TEQHRGC/MS/L and 4.97 pg-TEQCALUX/L. For soil samples, HRGC/MS-TEQ values ranged from 0 to 47.18 pg-TEQ/g (dry) and correlated well (r2 = 0.98) with values obtained by CALUX-TEQ which ranged from 0.92 to 649.97 pg-TEQ/g (dry). The difference between the absolute TEQ values might be due to the presence of dioxin-like compounds without WHO-TEQ values rather than the difference between CALUX-REP and WHO-TEQ. Based on this study, we suggest that DRE-CALUX bioassay can serve as an alternative bioassay method for high-throughput analysis of large number of environmental samples. © 2006 Elsevier B.V. All rights reserved.
DOI
10.1016/j.scitotenv.2006.10.036
Appears in Collections:
약학대학 > 약학과 > Journal papers
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