Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 배윤수 | * |
dc.date.accessioned | 2017-02-15T08:02:47Z | - |
dc.date.available | 2017-02-15T08:02:47Z | - |
dc.date.issued | 2006 | * |
dc.identifier.issn | 0008-6363 | * |
dc.identifier.other | OAK-3705 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/234184 | - |
dc.description.abstract | Objective: We investigated the role of NADPH oxidase 4 (Nox4) on lipopolysaccharide (LPS)-induced proinflammatory responses by human aortic endothelial cells (HAECs). Methods and results: Yeast two-hybrid and glutathione-S-transferase pull-down assays indicated that the cytosolic Toll/IL-1R region of Toll-like receptor 4 (TLR4) (amino acids 739-769) is the responsible domain for interaction with the COOH terminal of Nox4 (amino acids 451-530). Consistently, overexpression of the COOH-terminal region of Nox4 inhibited nuclear factor-κB activation in response to LPS. Downregulation of Nox4 by transfection of siRNA specific to Nox4 in HAECs resulted in a failure to induce reactive oxygen species (ROS) generation and subsequent expression of intercellular adhesion molecule-1 (ICAM-1) and chemokines such as IL-8 and monocyte chemoattractant protein-1 (MCP-1) in response to LPS. Furthermore, transient transfection of endothelial cells with Nox4 siRNA led to a decrease in migration and adhesion of monocytes in response to LPS by 36% and 52%, respectively. Conclusions: Nox4 plays a central role in LPS-induced proinflammatory responses by endothelial cells in an ROS-dependent manner. © 2006 European Society of Cardiology. | * |
dc.language | English | * |
dc.title | Role of NADPH oxidase 4 in lipopolysaccharide-induced proinflammatory responses by human aortic endothelial cells | * |
dc.type | Article | * |
dc.relation.issue | 3 | * |
dc.relation.volume | 72 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 447 | * |
dc.relation.lastpage | 455 | * |
dc.relation.journaltitle | Cardiovascular Research | * |
dc.identifier.doi | 10.1016/j.cardiores.2006.09.012 | * |
dc.identifier.wosid | WOS:000242692300012 | * |
dc.identifier.scopusid | 2-s2.0-33750519444 | * |
dc.author.google | Park H.S. | * |
dc.author.google | Chun J.N. | * |
dc.author.google | Jung H.Y. | * |
dc.author.google | Choi C. | * |
dc.author.google | Bae Y.S. | * |
dc.contributor.scopusid | 배윤수(15031067200) | * |
dc.date.modifydate | 20240415133331 | * |