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Inhibition of receptor internalization attenuates the TNFα-induced ROS generation in non-phagocytic cells

Title
Inhibition of receptor internalization attenuates the TNFα-induced ROS generation in non-phagocytic cells
Authors
Woo C.-H.Kim T.-H.Choi J.-A.Ryu H.-C.Lee J.E.You H.-J.Bae Y.-S.Kim J.-H.
Ewha Authors
배윤수
SCOPUS Author ID
배윤수scopus
Issue Date
2006
Journal Title
Biochemical and Biophysical Research Communications
ISSN
0006-291XJCR Link
Citation
vol. 351, no. 4, pp. 972 - 978
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Reactive oxygen species (ROS) are important regulatory molecules implicated in the signaling cascade triggered by tumor necrosis factor (TNF)α, although the events through which TNFα induces ROS generation are not well characterized. Here, we report that TNFα-induced ROS production was blocked by pretreatment with internalization inhibitor monodansyl cadaverine (MDC). Similarly, a transient expression of a GTP-binding and hydrolysis-defective dynamin mutant (dynamin K44A) that had been shown to be defective in internalization significantly attenuated the TNFα-induced intracellular ROS production. Importantly, the inhibition of receptor internalization suppressed TNFα signaling to mitogen-activated protein kinases (MAPKs) stimulation. Together, our results suggest that receptor internalization is somehow necessary for the TNFα-induced ROS generation and subsequent intracellular downstream signaling in non-phagocytes. © 2006 Elsevier Inc. All rights reserved.
DOI
10.1016/j.bbrc.2006.10.154
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자연과학대학 > 생명과학전공 > Journal papers
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