Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 이경림 | * |
dc.date.accessioned | 2017-02-15T08:02:39Z | - |
dc.date.available | 2017-02-15T08:02:39Z | - |
dc.date.issued | 2006 | * |
dc.identifier.issn | 0898-6568 | * |
dc.identifier.other | OAK-3637 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/234146 | - |
dc.description.abstract | We previously reported that phosphorylated cofilin-triosephosphate isomerase (TPI) complex interacts with Na,K-ATPase and enhances the pump activity through the phosphorylation of cofilin via Rho-mediated signaling pathway. In this study, we tested the hypothesis that the dephosphorylation of cofilin may be induced through Na,K-ATPase inhibition by ouabain. The phosphorylation level of cofilin by ouabain which decreases in a time- and dose-dependent manner in various human cell lines, remains unchanged by pretreatment with Src inhibitor, PP2; epidermal growth factor receptor (EGFR) inhibitor, AG1478; Raf-1 kinase (Raf) inhibitor, GW5074; and ERK kinase (MEK) inhibitor, PD98059, and by transfection of Ras dominant negative mutant (RasN17). This suggests that ouabain dephosphorylates cofilin through the Src/EGFR/Ras/Raf/MEK pathway. Ouabain activates Ras/Raf/MEK pathway, but down-regulates Rho kinase (ROCK)/LIM kinase (LIMK)/cofilin pathway, implying that there may be a cross-talk by ouabain between the Ras/Raf/MEK and the ROCK/LIMK/cofilin pathways. Immunofluorescence and flow cytometry suggest that ouabain-induced active form of cofilin may be involved in cytoskeletal reorganization and cell volume regulation. Thus, these findings demonstrate a new molecular mechanism for the dephosphorylation of cofilin through the inhibition of Na,K-ATPase by ouabain. © 2006 Elsevier Inc. All rights reserved. | * |
dc.language | English | * |
dc.title | Molecular mechanism of cofilin dephosphorylation by ouabain | * |
dc.type | Article | * |
dc.relation.issue | 11 | * |
dc.relation.volume | 18 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 2033 | * |
dc.relation.lastpage | 2040 | * |
dc.relation.journaltitle | Cellular Signalling | * |
dc.identifier.doi | 10.1016/j.cellsig.2006.03.014 | * |
dc.identifier.wosid | WOS:000241717900023 | * |
dc.identifier.scopusid | 2-s2.0-33749267063 | * |
dc.author.google | Jung J. | * |
dc.author.google | Kim M. | * |
dc.author.google | Choi S. | * |
dc.author.google | Kim M.-J. | * |
dc.author.google | Suh J.-k. | * |
dc.author.google | Choi E.C. | * |
dc.author.google | Lee K. | * |
dc.contributor.scopusid | 이경림(7501517435) | * |
dc.date.modifydate | 20240301081003 | * |