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Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DsrEFH from Allochromatium vinosum

Title
Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DsrEFH from Allochromatium vinosum
Authors
Dahl C.Schulte A.Shin D.H.
Ewha Authors
신동해
SCOPUS Author ID
신동해scopus
Issue Date
2007
Journal Title
Acta Crystallographica Section F: Structural Biology and Crystallization Communications
ISSN
1744-3091JCR Link
Citation
vol. 63, no. 10, pp. 890 - 892
Indexed
SCOPUS WOS scopus
Abstract
In purple sulfur bacteria, the proteins encoded by dsr genes play an essential role in the oxidation of intracellular sulfur, which is an obligate intermediate during the oxidation of sulfide and thiosulfate. One such gene product, DsrEFH from Allochromatium vinosum, has been cloned, expressed, purified and crystallized. Synchrotron data were collected to 2.5 Å from a crystal of selenomethionine-substituted DsrEFH. The crystal belongs to the primitive monoclinic space group P21, with unit-cell parameters a = 56.6, b = 183.1, c = 107.8 Å, β = 99.6°. A full structure determination is under way in order to provide insight into the structure-function relationships of this protein. © International Union of Crystallography 2007.
DOI
10.1107/S1744309107041188
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약학대학 > 약학과 > Journal papers
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