NL repository
menu
검색
Library
Browse
Communities & Collections
By Date
Authors
Titles
Subject
My Repository
My Account
Receive email updates
Edit Profile
DSpace at EWHA
자연과학대학
화학·나노과학전공
Journal papers
View : 529 Download: 0
Erythrocyte ghost-mediated gene delivery for prolonged and blood-targeted expression
Title
Erythrocyte ghost-mediated gene delivery for prolonged and blood-targeted expression
Authors
Byun H.-M.
;
Suh D.
;
Yoon H.
;
Kim J.M.
;
Choi H.-G.
;
Kim W.-K.
;
Ko J.J.
;
Oh Y.-K.
Ewha Authors
김원기
SCOPUS Author ID
김원기
Issue Date
2004
Journal Title
Gene Therapy
ISSN
0969-7128
Citation
Gene Therapy vol. 11, no. 5, pp. 492 - 496
Indexed
SCI; SCIE; SCOPUS
Document Type
Article
Abstract
This study reports the use of erythrocyte ghosts (EG) as a biocompatible nonviral delivery system for extended circulation and prolonged expression of plasmid DNA in the blood. Murine interleukin-2-expressing plasmid DNA was efficiently loaded to EG by electroporation in hypotonic condition. The presence of plasmid DNA in EG was confirmed by fluorescence-labeled plasmid DNA. At 21 min after intravenous administration into mice, the level of plasmid DNA in the blood was 92 000-fold higher following EG-mediated delivery as compared to the injection of naked form. EG-mediated gene delivery revealed higher and more prolonged mRNA expression levels of plasmid DNA in the blood until 9 days after the single intravenous injection. Moreover, plasmid DNA-loaded EG showed gene expression targeted to the blood cells. At 3 days post-dose, substantial expression levels of plasmid DNA delivered in EG were observed only in the blood and not in the other organs. Of the blood cells, the subpopulation containing granulocytes showed higher expression of plasmid DNA than mononuclear cells. These results indicate the potential of EG as a safe, prolonged and blood-targeted delivery system of therapeutic genes. © 2004 Nature Publishing Group. All rights reserved.
DOI
10.1038/sj.gt.3302180
Appears in Collections:
자연과학대학
>
화학·나노과학전공
>
Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML
Show full item record
Find@EWHA
트윗하기
BROWSE
Communities & Collections
By Date
Authors
Titles
Subject