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Erythrocyte ghost-mediated gene delivery for prolonged and blood-targeted expression

Title
Erythrocyte ghost-mediated gene delivery for prolonged and blood-targeted expression
Authors
Byun H.-M.Suh D.Yoon H.Kim J.M.Choi H.-G.Kim W.-K.Ko J.J.Oh Y.-K.
Ewha Authors
김원기
Issue Date
2004
Journal Title
Gene Therapy
ISSN
0969-7128JCR Link
Citation
vol. 11, no. 5, pp. 492 - 496
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
This study reports the use of erythrocyte ghosts (EG) as a biocompatible nonviral delivery system for extended circulation and prolonged expression of plasmid DNA in the blood. Murine interleukin-2-expressing plasmid DNA was efficiently loaded to EG by electroporation in hypotonic condition. The presence of plasmid DNA in EG was confirmed by fluorescence-labeled plasmid DNA. At 21 min after intravenous administration into mice, the level of plasmid DNA in the blood was 92 000-fold higher following EG-mediated delivery as compared to the injection of naked form. EG-mediated gene delivery revealed higher and more prolonged mRNA expression levels of plasmid DNA in the blood until 9 days after the single intravenous injection. Moreover, plasmid DNA-loaded EG showed gene expression targeted to the blood cells. At 3 days post-dose, substantial expression levels of plasmid DNA delivered in EG were observed only in the blood and not in the other organs. Of the blood cells, the subpopulation containing granulocytes showed higher expression of plasmid DNA than mononuclear cells. These results indicate the potential of EG as a safe, prolonged and blood-targeted delivery system of therapeutic genes. © 2004 Nature Publishing Group. All rights reserved.
DOI
10.1038/sj.gt.3302180
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자연과학대학 > 화학·나노과학전공 > Journal papers
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