View : 20 Download: 0

Glucose Deprivation Increases Hydrogen Peroxide Level in Immunostimulated Rat Primary Astrocytes

Title
Glucose Deprivation Increases Hydrogen Peroxide Level in Immunostimulated Rat Primary Astrocytes
Authors
Choi J.W.Shin C.Y.Yoo B.K.Choi M.S.Lee W.J.Han B.H.Kim W.-K.Kim H.-C.Ko K.H.
Ewha Authors
김원기
Issue Date
2004
Journal Title
Journal of Neuroscience Research
ISSN
0360-4012JCR Link
Citation
vol. 75, no. 5, pp. 722 - 731
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Activated astrocytes produce a large amount of bioactive molecules, including reactive oxygen and nitrogen species. Astrocytes are in general resistant to those reactive species. However, we previously reported that immunostimulated astrocytes became highly vulnerable to metabolic insults, such as glucose deprivation. In this study, we investigated whether H 2O2 production was associated with the increased vulnerability. Glucose deprivation for up to 8 hr did not change the intracellular level of H2O2 in astrocytes. Treatment with lipopolysaccharide plus interferon-γ for 48 hr evoked astroglial H 2O2 production; however, no apparent death or injury was observed in immunostimulated astrocytes. Glucose deprivation after 48 hr of immunostimulation markedly increased H2O2 level, depleted adenosine triphosphate (ATP), and enhanced lactate dehydrogenase (LDH) release. The ATP depletion and LDH release were in part prevented by catalase, mannitol, and N-acetyl-L-cysteine. The enhanced level of H2O2 in glucose-deprived immunostimulated astrocytes appeared to be secondary to the depletion of reduced glutathione. 4-(2-Aminoethyl)bebzenesulfonyl fluoride (AEBSF), an inhibitor of NADPH oxidase, reduced H2O2 level and LDH release in glucose-deprived immunostimulated astrocytes. H 2O2, either endogenously produced or exogenously added, depolarized mitochondrial transmembrane potential in glucose-deprived astrocytes, leading to their ATP depletion and death. The present results strongly indicate that glucose deprivation causes deterioration of immunostimulated astrocytes by increasing the intracellular concentration of H2O2. © 2004 Wiley-Liss, Inc.
DOI
10.1002/jnr.20009
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE