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Toxic effects of carbendazim and n-butyl isocyanate, metabolites of the fungicide benomyl, on early development in the African clawed frog, Xenopus laevis

Title
Toxic effects of carbendazim and n-butyl isocyanate, metabolites of the fungicide benomyl, on early development in the African clawed frog, Xenopus laevis
Authors
Yoon C.-S.Jin J.-H.Park J.-H.Yeo C.-Y.Kim S.-J.Hwang Y.-G.Hong S.-J.Cheong S.-W.
Ewha Authors
여창열
SCOPUS Author ID
여창열scopus
Issue Date
2008
Journal Title
Environmental Toxicology
ISSN
1520-4081JCR Link
Citation
vol. 23, no. 1, pp. 131 - 144
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
We investigated the toxic effects of carbendazim and n-butyl isocyanate (BIC), metabolites of the fungicide benomyl, on development in the African clawed frog, Xenopus laevis. To test the toxic effects, frog embryo teratogenesis assays using Xenopus were performed. Embryos were exposed to various concentrations of carbendazim (0-7 μM) and BIC (0-0.2 μM). LC 100 for carbendazim and BIC were 7 and 0.2 μM, respectively, and the corresponding LC 50, determined by probit analysis, were 5.606 and 0.135 μM. Exposure to carbendazim concentrations ≥3 μM and BIC concentrations ≥0.1 μM resulted in 10 different types of severe external malformation. Histological examinations revealed dysplasia of the brain, eyes, intestine, and somatic muscle, and swelling of the pronephric ducts. These phenomena were common in both test groups. The tissue-specific toxic effects were investigated with an animal cap assay. Neural tissues are normally induced at a high frequency by activin A, however, the induction of neural tissues was strongly inhibited by the addition of carbendazim. Conversely, the addition of BIC resulted in weak inhibition of neural tissues. Electron micrographs of animal cap explants revealed degeneration of cell junctions in the carbendazim-treated group, but not in the BIC-treated group. Numerous residual yolk platelets and mitochondrial degeneration were commonly observed in both test groups. The gene expression of cultivated animal cap explants was investigated by reverse transcriptase-polymerase chain reaction and revealed that expression of the neural-specific marker neural cell adhesion molecule was more strongly inhibited in the carbendazim-treated group than in the BIC-treated group. © 2008 Wiley Periodicals, Inc.
DOI
10.1002/tox.20338
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자연과학대학 > 생명과학전공 > Journal papers
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