Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이승진 | * |
dc.contributor.author | 조인호 | * |
dc.date.accessioned | 2017-01-05T02:01:58Z | - |
dc.date.available | 2017-01-05T02:01:58Z | - |
dc.date.issued | 2008 | * |
dc.identifier.issn | 0898-6568 | * |
dc.identifier.other | OAK-4722 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/233540 | - |
dc.description.abstract | The intracellular signaling events controlling human mesenchymal stem cell (hMSC) differentiation into osteoblasts are poorly understood. Collagen-binding domain is considered an essential component of bone mineralization. In the present study, we investigated the regulatory mechanism of osteoblastic differentiation of hMSC by the peptide with a novel collagen-binding motif derived from osteopontin. The peptide induced influx of extracellular Ca2+ via calcium channels and increased intracellular Ca2+ concentration ([Ca2+]i) independent of both pertussis toxin and phospholipase C, and activated ERK, which was inhibited by Ca2+/calmodulin-dependent protein kinase (CaMKII) antagonist, KN93. The peptide-induced increase of [Ca2+]i is correlated with ERK activation in a various cell types. The peptide stimulated the migration of hMSC but suppressed cell proliferation. Furthermore, the peptide increased the phosphorylation of cAMP-response element-binding protein, leading to a significant increase in the transactivation of cAMP-response element and serum response element. Ultimately, the peptide increased AP-1 transactivation, c-jun expression, and bone mineralization, which are suppressed by KN93. Taken together, these results indicate that the novel collagen-binding peptide promotes osteogenic differentiation via Ca2+/CaMKII/ERK/AP-1 signaling pathway in hMSC, suggesting the potential application in cell therapy for bone regeneration. © 2007 Elsevier Inc. All rights reserved. | * |
dc.language | English | * |
dc.title | A novel collagen-binding peptide promotes osteogenic differentiation via Ca2+/calmodulin-dependent protein kinase II/ERK/AP-1 signaling pathway in human bone marrow-derived mesenchymal stem cells | * |
dc.type | Article | * |
dc.relation.issue | 4 | * |
dc.relation.volume | 20 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 613 | * |
dc.relation.lastpage | 624 | * |
dc.relation.journaltitle | Cellular Signalling | * |
dc.identifier.doi | 10.1016/j.cellsig.2007.11.012 | * |
dc.identifier.wosid | WOS:000254725300004 | * |
dc.identifier.scopusid | 2-s2.0-39249085796 | * |
dc.author.google | Shin M.K. | * |
dc.author.google | Kim M.-K. | * |
dc.author.google | Bae Y.-S. | * |
dc.author.google | Jo I. | * |
dc.author.google | Lee S.-J. | * |
dc.author.google | Chung C.-P. | * |
dc.author.google | Park Y.-J. | * |
dc.author.google | Min D.S. | * |
dc.contributor.scopusid | 이승진(57196249292) | * |
dc.contributor.scopusid | 조인호(26643129000;56663841900) | * |
dc.date.modifydate | 20240123112949 | * |