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Functional and developmental analysis of the blood-brain barrier in zebrafish

Title
Functional and developmental analysis of the blood-brain barrier in zebrafish
Authors
Jeong J.-Y.Kwon H.-B.Ahn J.-C.Kang D.Kwon S.-H.Park J.A.Kim K.-W.
Ewha Authors
강동민
SCOPUS Author ID
강동민scopus
Issue Date
2008
Journal Title
Brain Research Bulletin
ISSN
0361-9230JCR Link
Citation
vol. 75, no. 5, pp. 619 - 628
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The blood-brain barrier (BBB) is essential for maintaining brain homeostasis and protecting the brain from toxic substances. Breakdown of this barrier results in severe brain pathologies, whereas impermeability of the BBB is a major obstacle for drug delivery to the brain. Despite its importance, our understanding of the maturation and modulation of the BBB is limited. Zebrafish (Danio rerio) has emerged as a useful model organism for studying vertebrate development and disease mechanisms, as well as for preclinical drug screening. However, the nature of the BBB has not yet been examined in teleost fish. In this paper, we report that with the exception of the circumventricular organs, the cerebral microvessels in zebrafish are impermeable to sulfo-NHS-biotin and horseradish peroxidase (HRP). Brain endothelial cells show immunoreactivity to Claudin-5 and Zonula Occludens-1 (ZO-1), implying the presence of tight junctions in these cells. The expression of Claudin-5 and ZO-1 was detected in cerebral microvessels from 3 days post-fertilization (dpf), concomitant with maturation of the BBB, as determined by restricted permeability to HRP and various fluorescent tracers. Real-time analysis of fluorescent tracer leakage in embryonic zebrafish suggests that they may be used as an in vivo model for BBB breakdown. Taken together, our results show that the endothelial tight junction-based BBB of zebrafish is similar to that of higher vertebrates and thus, zebrafish may be an excellent genetic and experimental model organism for studying development and maintenance of the BBB. © 2007 Elsevier Inc. All rights reserved.
DOI
10.1016/j.brainresbull.2007.10.043
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자연과학대학 > 생명과학전공 > Journal papers
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