Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 김진흥 | - |
dc.date.accessioned | 2017-01-05T02:01:55Z | - |
dc.date.available | 2017-01-05T02:01:55Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0916-8451 | - |
dc.identifier.other | OAK-4779 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/233513 | - |
dc.description.abstract | α and β tubulin genes were cloned from the Capsicum annuum leaves using rapid amplification of cDNA ends (RACE)-PCR. Nucleotide sequence analysis revealed that 1,353 bp Capsicum annuum α/β-tubulin (CAnm α/β-TUB) encodes a protein of 450 amino acids (aa) each. The recombinant α/β tubulin was overexpressed mainly as an inclusion body in Escherichia coli BL21 (DE3), upon induction with 0.2 mM isopropyl-β-D- thiogalactopyranoside (IPTG), and its content was as high as 50% of the total protein content. Effective fusion protein purification and refolding are described. The average yields of α and β tubulin were 2.0 and 1.3 mg/l of culture respectively. The apparent molecular weight of each tubulin was estimated to be 55 kDa by SDS-polyacrylamide gel electrophoresis (PAGE). The tubulin monomers were found to be assembly competent using a standard dimerization assay, and also retained antigenicity with anti-His/T7 antibodies. The purified tubulins were polymerized to microtubule-like structures in the presence of 2 mM guanosine 5′-triphosphate (GTP). | - |
dc.language | English | - |
dc.title | Cloning, purification, and polymerization of Capsicum annuum recombinant α and β tubulin | - |
dc.type | Article | - |
dc.relation.issue | 4 | - |
dc.relation.volume | 72 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 1048 | - |
dc.relation.lastpage | 1055 | - |
dc.relation.journaltitle | Bioscience, Biotechnology and Biochemistry | - |
dc.identifier.doi | 10.1271/bbb.70794 | - |
dc.identifier.wosid | WOS:000255501100017 | - |
dc.identifier.scopusid | 2-s2.0-43549125726 | - |
dc.author.google | Jang M.-H. | - |
dc.author.google | Kim J. | - |
dc.author.google | Kalme S. | - |
dc.author.google | Han J.-W. | - |
dc.author.google | Yoo H.-S. | - |
dc.author.google | Koo B.-S. | - |
dc.author.google | Kim S.-K. | - |
dc.author.google | Yoon M.-Y. | - |
dc.contributor.scopusid | 김진흥(8580015800) | - |
dc.date.modifydate | 20170601134213 | - |