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Ionic mechanisms of desflurane on prolongation of action potential duration in rat ventricular myocytes

Title
Ionic mechanisms of desflurane on prolongation of action potential duration in rat ventricular myocytes
Authors
Chae J.E.Kim H.S.Ahn D.S.Park W.K.
Ewha Authors
김현수
Issue Date
2012
Journal Title
Yonsei Medical Journal
ISSN
0513-5796JCR Link
Citation
vol. 53, no. 1, pp. 204 - 212
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
Purpose: Despite the fact that desflurane prolongs the QTC interval in humans, little is known about the mechanisms that underlie these actions. We investigated the effects of desflurane on action potential (AP) duration and underlying electrophysiological mechanisms in rat ventricular myocytes. Materials and Methods: Rat ventricular myocytes were enzymatically isolated and studied at room temperature. AP was measured using a current clamp technique. The effects of 6% (0.78 mM) and 12% (1.23 mM) desflurane on transient outward K + current (Ito), sustained outward current (Isus), inward rectifier K+ current (IKI), and L-type Ca 2+ current were determined using a whole cell voltage clamp. Results: Desflurane prolonged AP duration, while the amplitude and resting membrane potential remained unchanged. Desflurane at 0.78 mM and 1.23 mM significantly reduced the peak Ito by 20±8% and 32±7%, respectively, at +60 mV. Desflurane (1.23 mM) shifted the steady-state inactivation curve in a hyperpolarizing direction and accelerated inactivation of the current. While desflurane (1.23 mM) had no effects on Isus and IKI, it reduced the L-type Ca 2+ current by 40±6% (p<0.05). Conclusion: Clinically relevant concentrations of desflurane appear to prolong AP duration by suppressing Ito in rat ventricular myocytes. © Yonsei University College of Medicine 2012.
DOI
10.3349/ymj.2012.53.1.204
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자연과학대학 > 생명과학전공 > Journal papers
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