Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 박진병 | * |
dc.date.accessioned | 2016-12-27T02:12:22Z | - |
dc.date.available | 2016-12-27T02:12:22Z | - |
dc.date.issued | 2016 | * |
dc.identifier.issn | 2155-5435 | * |
dc.identifier.other | OAK-19863 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/233136 | - |
dc.description.abstract | Oxyfunctionalization of plant oils such as olive oil and soybean oil into C9 carboxylic acids (e.g., n-nonanoic acid and 9-hydroxynonanoic acid) was investigated. The biotransformation was composed of hydrolysis of plant oils by the Thermomyces lanuginostis lipase (TLL) and C9-C10 double-bond cleavage in unsaturated fatty acids by a serial reaction of a fatty acid double bond-hydratase of Stenotrophomonas maltophilia, an alcohol dehydrogenase of Micrococcus luteus, and a Baeyer-Villiger monooxygenase (BVMO) of Pseudomonas putida KT2440 expressed in Escherichia coli. The newly cloned oleate hydratase allowed one to produce 10-hydroxyoctadecanoic acid and 10-hydroxyoctadec-12-enoic acid at a high rate from oleic acid and linoleic acid, respectively, which are major fatty acid constituents of many plant oils. Furthermore, overexpression of a long chain fatty acid transporter FadL in the recombinant E. coli led to a significant increase of whole-cell biotransformation rates of oleic acid and linoleic acid into the corresponding esters. The resulting esters (the BVMO reaction products) were hydrolyzed in situ by TLL, generating nonanoic acid, non-3-enoic acid, and 9-hydroxynonanoic acid, which can be further oxidized to 1,9-nonanedioic acid. This study demonstrated that industrially relevant C9 carboxylic acids could be produced from olive oil or soybean oil by simultaneous enzyme/whole-cell biocatalysis. | * |
dc.language | English | * |
dc.publisher | AMER CHEMICAL SOC | * |
dc.subject | oleate hydratase | * |
dc.subject | FadL | * |
dc.subject | 9-hydroxynonanoic acid | * |
dc.subject | olive oil | * |
dc.subject | soybean oil | * |
dc.subject | simultaneous enzyme/whole-cell biocatalysis | * |
dc.title | Simultaneous Enzyme/Whole-Cell Biotransformation of Plant Oils into C9 Carboxylic Acids | * |
dc.type | Article | * |
dc.relation.issue | 11 | * |
dc.relation.volume | 6 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 7547 | * |
dc.relation.lastpage | 7553 | * |
dc.relation.journaltitle | ACS CATALYSIS | * |
dc.identifier.doi | 10.1021/acscatal.6b01884 | * |
dc.identifier.wosid | WOS:000387306100030 | * |
dc.author.google | Jeon, Eun-Yeong | * |
dc.author.google | Seo, Joo-Hyun | * |
dc.author.google | Kang, Woo-Ri | * |
dc.author.google | Kim, Min-Ji | * |
dc.author.google | Lee, Jung-Hoo | * |
dc.author.google | Oh, Deok-Kun | * |
dc.author.google | Park, Jin-Byung | * |
dc.contributor.scopusid | 박진병(15036390700) | * |
dc.date.modifydate | 20240322114808 | * |