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The tobacco gene Ntcyc07 confers arsenite tolerance in Saccharomyces cerevisiae by reducing the steady state levels of intracellular arsenic

Title
The tobacco gene Ntcyc07 confers arsenite tolerance in Saccharomyces cerevisiae by reducing the steady state levels of intracellular arsenic
Authors
Mok Y.G.Lee B.D.Kim Y.J.Lee C.E.Kim D.G.Lee J.Shim J.Meng Y.Rosen B.P.Choi J.S.Shin H.S.Kim S.-K.Lee J.S.Hwang S.
Ewha Authors
이준승
SCOPUS Author ID
이준승scopusscopus
Issue Date
2008
Journal Title
FEBS Letters
ISSN
0014-5793JCR Link
Citation
vol. 582, no. 6, pp. 916 - 924
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
We cloned a plant gene, Ntcyc07, conferring arsenite tolerance by expressing a tobacco expression library in WT yeast (Y800). Expression of Ntcyc07 increased the tolerance to As(III) and decreased its accumulation, suggesting that the enhanced As(III) tolerance resulted from a reduction of the intracellular arsenic level. Interestingly, expression of Ntcyc07 increased the expression of the As(III) export carrier ACR3, but repressed that of As(III) uptake channel FPS1. Ntcyc07p interacted with Acr1p, which is the transcriptional activator of ACR3, but not with the ACR3 promoter. Taken together, the data indicated that Ntcyc07p promoted As(III) tolerance by decreasing the intracellular level of As(III) via increasing the expression of ACR3 and reducing that of FPS1. © 2008 Federation of European Biochemical Societies.
DOI
10.1016/j.febslet.2008.02.030
Appears in Collections:
자연과학대학 > 생명과학전공 > Journal papers
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