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A rapid and sensitive method for detecting foodborne pathogens by capillary electrophoresis-based single-strand conformation polymorphism

Title
A rapid and sensitive method for detecting foodborne pathogens by capillary electrophoresis-based single-strand conformation polymorphism
Authors
Oh M.-H.Park Y.S.Paek S.-H.Kim H.-Y.Jung G.Y.Oh S.
Ewha Authors
오상석
SCOPUS Author ID
오상석scopus
Issue Date
2008
Journal Title
Food Control
ISSN
0956-7135JCR Link
Citation
vol. 19, no. 11, pp. 1100 - 1104
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
A parallel analysis method using capillary electrophoresis-based single-strand conformation polymorphism (CE-SSCP) combined with 16S rRNA gene-specific PCR was developed for rapid and effective detection of five pathogens associated with food poisoning. Conventional detection and identification methods for these pathogens are time consuming and labor intensive due to the necessity of separate cultivation of each target strain. Using the developed CE-SSCP method, Escherichia coli, Clostridium perfringens, Campylobacter jejuni, Salmonella enterica, and Bacillus cereus were simultaneously identified with individual corresponding peaks from a mixture of their genomic DNA. This method can be used for rapid investigation of causative agents of food poisoning. Its simplicity and high sensitivity may lead to improved management of food safety. © 2007 Elsevier Ltd. All rights reserved.
DOI
10.1016/j.foodcont.2007.11.009
Appears in Collections:
엘텍공과대학 > 식품공학전공 > Journal papers
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