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Nanoscale observation of local bound charges of patterned protein arrays by scanning force microscopy

Title
Nanoscale observation of local bound charges of patterned protein arrays by scanning force microscopy
Authors
Oh Y.J.Jo W.Kim S.Park S.Kim Y.S.
Ewha Authors
조윌렴박성수
SCOPUS Author ID
조윌렴scopus
Issue Date
2008
Journal Title
Nanotechnology
ISSN
0957-4484JCR Link
Citation
vol. 19, no. 36
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
A protein patterned surface using micro-contact printing methods has been investigated by scanning force microscopy. Electrostatic force microscopy (EFM) was utilized for imaging the topography and detecting the electrical properties such as the local bound charge distribution of the patterned proteins. It was found that the patterned IgG proteins are arranged down to 1 μm, and the 90° rotation of patterned anti-IgG proteins was successfully undertaken. Through the estimation of the effective areas, it was possible to determine the local bound charges of patterned proteins which have opposite electrostatic force behaviors. Moreover, we studied the binding probability between IgG and anti-IgG in a 1 μm 2 MIMIC system by topographic and electrostatic signals for applicable label-free detections. We showed that the patterned proteins can be used for immunoassay of proteins on the functional substrate, and that they can also be used for bioelectronics device application, indicating distinct advantages with regard to accuracy and a label-free detection. © IOP Publishing Ltd.
DOI
10.1088/0957-4484/19/36/365302
Appears in Collections:
자연과학대학 > 물리학전공 > Journal papers
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