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Cellular uptake and cytotoxicity of octahedral rhenium cluster complexes

Title
Cellular uptake and cytotoxicity of octahedral rhenium cluster complexes
Authors
Choi S.-J.Brylev K.A.Xu J.-Z.Mironov Y.V.Fedorov V.E.Sohn Y.S.Kim S.-J.Choy J.-H.
Ewha Authors
김성진손연수최진호
SCOPUS Author ID
김성진scopus; 손연수scopus; 최진호scopus
Issue Date
2008
Journal Title
Journal of Inorganic Biochemistry
ISSN
0162-0134JCR Link
Citation
vol. 102, no. 11, pp. 1991 - 1996
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Cellular uptake behavior of a novel class of octahedral rhenium cluster compounds, hexahydroxo complexes K4[{Re6S8}(OH)6] · 8H2O (1) and K4[{Re6Se8}(OH)6] · 8H2O (2), was evaluated in human cervical adenocarcinoma HeLa cells. Confocal microscopy and flow cytometry studies demonstrated that rhenium cluster 1 was not internalized into cell, while rhenium cluster 2 was. Conjugation of a polymer to rhenium cluster 1, namely the derivative K4[{Re6S8}(OH)5L] (3) (L is amphiphilic diblock copolymer MPEG550-CH2CONH-GlyPheLeuGlyPheLeu-COO-), considerably enhanced cellular uptake in a concentration-dependent manner and was predominantly localized in the cytoplasm and nucleus upon incubation time. The uptake of rhenium cluster 2 was mediated by energy-dependent endocytosis, whereas rhenium cluster 3 was directly ingested into cells by cell-fusion-like mechanism. According to the cytotoxicity evaluation test, both rhenium clusters 2 and 3 did not exhibit acute cytotoxic effects up to 50 μM, at the practical concentration level of biological applications. It is, therefore, expected that the rhenium cluster complexes can be promising potential candidates as diagnostic agents for medical treatment. © 2008 Elsevier Inc. All rights reserved.
DOI
10.1016/j.jinorgbio.2008.07.013
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자연과학대학 > 화학·나노과학전공 > Journal papers
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