View : 11 Download: 0

Synthetic peptide-coated bone mineral for enhanced osteoblastic activation in vitro and in vivo

Title
Synthetic peptide-coated bone mineral for enhanced osteoblastic activation in vitro and in vivo
Authors
Lee J.-Y.Choo J.-E.Park H.-J.Park J.-B.Lee S.-C.Lee S.-J.Park Y.-J.Chung C.-P.
Ewha Authors
이승진
SCOPUS Author ID
이승진scopus
Issue Date
2008
Journal Title
Journal of Biomedical Materials Research - Part A
ISSN
1549-3296JCR Link
Citation
vol. 87, no. 3, pp. 688 - 697
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
A 15-mer synthetic peptide, designated P1, was derived from the bone morphogenetic protein (BMP) receptor I and BMP receptor II binding domains of BMP-2 for the purpose of enhancing bone regeneration capacity of inorganic bovine bone mineral. A second peptide, denoted P2, was designed by adding seven glutamic acid residues to the N-terminal of P1 to increase the surface coating efficiency onto bone mineral. The coating efficiency of P1 increased with the concentration of peptide. P2 peptide, in contrast, had a higher coating efficiency at lower peptide concentrations. The peptides properly transduced intracellular signals properly via the Smad and ERK pathways, thereby increasing mineralization in vitro, implying that the peptides alone can induce osteoblastic differentiation. Adhesion of cells to bone mineral was greater when peptides were present than in bone mineral alone. P1- and P2-coated bone mineral increased osteoblastic differentiation, as demonstrated by ALPase activity. P1-coated bone mineral stimulated more new bone regeneration in bone defect sites after 2 weeks than the peptide-free control. These peptides, in combination with bone grafts or implants, have the potential to enhance osteoblastic differentiation and bone regeneration. © 2008 Wiley Periodicals, Inc.
DOI
10.1002/jbm.a.31721
Appears in Collections:
약학대학 > 약학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE