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Secreted tryptophanyl-tRNA synthetase as a primary defence system against infection
- Secreted tryptophanyl-tRNA synthetase as a primary defence system against infection
- Ahn, Young Ha; Park, Sunyoung; Choi, Jeong June; Park, Bo-Kyung; Rhee, Kyung Hee; Kang, Eunjoo; Ahn, Soyeon; Lee, Chul-Ho; Lee, Jong Soo; Inn, Kyung-Soo; Cho, Mi-La; Park, Sung-Hwan; Park, Kyunghee; Park, Hye Jung; Lee, Jae-Hyun; Park, Jung-Won; Kwon, Nam Hoon; Shim, Hyunbo; Han, Byung Woo; Kim, Pilhan; Lee, Joo-Youn; Jeon, Youngho; Huh, Jin Won; Jin, Mirim; Kim, Sunghoon
- Ewha Authors
- SCOPUS Author ID
- Issue Date
- Journal Title
- NATURE MICROBIOLOGY
- vol. 2, no. 1
- NATURE PUBLISHING GROUP
- SCIE; SCOPUS
- The N-terminal truncated form of a protein synthesis enzyme, tryptophanyl-tRNA synthetase (mini-WRS), is secreted as an angiostatic ligand. However, the secretion and function of the full-length WRS (FL-WRS) remain unknown. Here, we report that the FL-WRS, but not mini-WRS, is rapidly secreted upon pathogen infection to prime innate immunity. Blood levels of FL-WRS were increased in sepsis patients, but not in those with sterile inflammation. FL-WRS was secreted from monocytes and directly bound to macrophages via a toll-like receptor 4 (TLR4)-myeloid differentiation factor 2 (MD2) complex to induce phagocytosis and chemokine production. Administration of FL-WRS into Salmonella typhimurium-infected mice reduced the levels of bacteria and improved mouse survival, whereas its titration with the specific antibody aggravated the infection. The N-terminal 154-amino-acid eukaryote-specific peptide of WRS was sufficient to recapitulate FL-WRS activity and its interaction mode with TLR4-MD2 is now suggested. Based on these results, secretion of FL-WRS appears to work as a primary defence system against infection, acting before full activation of innate immunity.
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