Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤여준 | - |
dc.date.accessioned | 2016-10-20T02:10:40Z | - |
dc.date.available | 2016-10-20T02:10:40Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.other | OAK-5393 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/232560 | - |
dc.description.abstract | Glycosyltransferases (GTs) are crucial enzymes in the biosynthesis and diversification of therapeutically important natural products, and the majority of them belong to the GT-B superfamily, which is composed of separate N- and C-domains that are responsible for the recognition of the sugar acceptor and donor, respectively. In an effort to expand the substrate specificity of GT, a chimeric library with different crossover points was constructed between the N-terminal fragments of kanamycin GT (kanF) and the C-terminal fragments of vancomycin GT (gtfE) genes by incremental truncation method. A plate-based pH color assay was newly developed for the selection of functional domain-swapped GTs, and a mutant (HMT31) with a crossover point (N-kanF-669 bp and 753 bp-gtfE-C) for domain swapping was screened. The most active mutant HMT31 (50 kDa) efficiently catalyzed 2-DOS (aglycone substrate for KanF) glucosylation using dTDP-glucose (glycone substrate for GtfE) with k cat/K m of 162.8 ± 0.1 mM -1 min -1. Moreover, HMT31 showed improved substrate specificity toward seven more NDP-sugars. This study presents a domain swapping method as a potential means to glyco- randomization toward various syntheses of 2-DOS-based aminoglycoside derivatives. © 2008 Wiley Periodicals, Inc. | - |
dc.language | English | - |
dc.title | Expanding substrate specificity of GT-B fold glycosyltransferase via domain swapping and high-throughput screening | - |
dc.type | Article | - |
dc.relation.issue | 4 | - |
dc.relation.volume | 102 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 988 | - |
dc.relation.lastpage | 994 | - |
dc.relation.journaltitle | Biotechnology and Bioengineering | - |
dc.identifier.doi | 10.1002/bit.22150 | - |
dc.identifier.wosid | WOS:000263135000003 | - |
dc.identifier.scopusid | 2-s2.0-58549102566 | - |
dc.author.google | Park S.-H. | - |
dc.author.google | Park H.-Y. | - |
dc.author.google | Sohng J.K. | - |
dc.author.google | Lee H.C. | - |
dc.author.google | Liou K. | - |
dc.author.google | Yoon Y.J. | - |
dc.author.google | Kim B.-G. | - |
dc.contributor.scopusid | 윤여준(7402126465) | - |
dc.date.modifydate | 20210708161345 | - |