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Efficient labeling of mesenchymal stem cells using cell permeable magnetic nanoparticles

Title
Efficient labeling of mesenchymal stem cells using cell permeable magnetic nanoparticles
Authors
Suh J.S.Lee J.Y.Choi Y.S.Yu F.Yang V.Lee S.J.Chung C.P.Park Y.J.
Ewha Authors
이승진
SCOPUS Author ID
이승진scopus
Issue Date
2009
Journal Title
Biochemical and Biophysical Research Communications
ISSN
0006-291XJCR Link
Citation
vol. 379, no. 3, pp. 669 - 675
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
For the purpose of successfully monitoring labeled cells, optimum labeling efficiency without any side effect is a prerequisite. Magnetic cellular imaging is a new and growing field that allows the visualization of implanted cells in vivo. Herein, superparamagnetic iron oxide (SPIO) nanoparticles were conjugated with a non-toxic protein transduction domain (PTD), identified by the authors and termed low molecular weight protamine (LMWP), to generate efficient and non-toxic cell labeling tools. The cells labeled with LMWP-SPIO presented the highest iron content compared to those labeled with naked SPIO and the complex of SPIO with poly-l-lysine, which is currently used as a transfection agent. In addition to the iron content assay, Prussian staining and confocal observation demonstrated the highest intracellular LMWP-SPIO presence, and the labeling procedure did not alter the cell differentiation capacity of mesenchymal stem cells. Taken together, cell permeable magnetic nanoparticles conjugated with LMWP can be suggested as labeling tools for efficient magnetic imaging of transplanted cells. © 2009.
DOI
10.1016/j.bbrc.2008.12.041
Appears in Collections:
약학대학 > 약학과 > Journal papers
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