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A novel small molecule, LAS-0811, inhibits alcohol-induced apoptosis in VL-17A cells

Title
A novel small molecule, LAS-0811, inhibits alcohol-induced apoptosis in VL-17A cells
Authors
Kim T.-H.Venugopal S.K.Zhu M.Wang S.-S.Lau D.Lam K.S.Clemens D.L.Zern M.A.
Ewha Authors
김태헌
SCOPUS Author ID
김태헌scopus
Issue Date
2009
Journal Title
Biochemical and Biophysical Research Communications
ISSN
0006-291XJCR Link
Citation
vol. 379, no. 4, pp. 876 - 881
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
One of the pathways by which alcohol induces hepatocyte apoptosis is via oxidative stress. We screened several chemically-synthesized small molecules and found LAS-0811, which inhibits oxidative stress. In this study, we elucidated its role in inhibiting alcohol-induced apoptosis in hepatocyte-like VL-17A cells. VL-17A cells were pre-incubated with LAS-0811, followed by ethanol incubation. Ethanol-induced reactive oxygen species and apoptosis were significantly inhibited in LAS-0811 pre-treated cells. VL-17A cells were transfected with a reporter (ARE/TK-GFP) plasmid containing green fluorescent protein (GFP) as a reporter gene and the anti-oxidant response element as the promoter. LAS-0811 pre-treatment significantly induced the GFP expression compared to the cells treated with ethanol alone. LAS-0811 induced the activation of nrf2 and enhanced the expression and activity of glutathione peroxidase, one of the downstream targets of nrf2. The results indicate that LAS-0811 protects VL-17A cells against ethanol-induced oxidative stress and apoptosis at least in part via nrf2 activation. © 2008 Elsevier Inc. All rights reserved.
DOI
10.1016/j.bbrc.2008.12.133
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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