Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 박성수 | - |
dc.date.accessioned | 2016-10-20T02:10:35Z | - |
dc.date.available | 2016-10-20T02:10:35Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.other | OAK-5472 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/232514 | - |
dc.description.abstract | Rapsyn is a postsynaptic protein required for clustering of nicotinic acetylcholine receptors (nAChRs) at the neuromuscular junction. Here we report the mechanism for posttranslational control of rapsyn protein stability. We confirmed that C18H9.7-encoded RPY-1 is a rapsyn homolog in Caenorhabditis elegans by showing that human rapsyn rescued rpy-1 mutant phenotypes in nematodes, as determined by levamisole assays and micropost array behavioral assays. We found that RPY-1 was degraded in the absence of functional UNC-29, a non-α subunit of the receptor, in an allele-specific manner, but not in the absence of other receptor subunits. The cytoplasmic loop of UNC-29 was found to be critical for RPY-1 stability. Through RNA interference screening, we found that UBC-1, UBC-12, NEDD-8, and RBX-1 were required for degradation of RPY-1. We identified cullin (CUL)-3 as a component of E3 ligase and KEL-8 as the substrate adaptor of RPY-1. Mammalian rapsyn was ubiquitinated by the CUL3/KLHL8-containing E3 ligase in vitro, and the knockdown of KLHL-8, a mammalian KEL-8 homolog, inhibited rapsyn ubiquitination in vivo, implying evolutionary conservation of the rapsyn stability control machinery. kel-8 suppression and rpy-1 overexpression in C. elegans produced a phenotype similar to that of a loss-of-function mutation of rpy-1, suggesting that control of rapsyn abundance is important for proper function of the receptor. Our results suggest a link between the control of rapsyn abundance and congenital myasthenic syndromes. © 2009 by The American Society for Biochemistry and Molecular Biology, Inc. | - |
dc.language | English | - |
dc.title | Control of rapsyn stability by the CUL-3-containing E3 ligase complex | - |
dc.type | Article | - |
dc.relation.issue | 12 | - |
dc.relation.volume | 284 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 8195 | - |
dc.relation.lastpage | 8206 | - |
dc.relation.journaltitle | Journal of Biological Chemistry | - |
dc.identifier.doi | 10.1074/jbc.M808230200 | - |
dc.identifier.wosid | WOS:000264195600081 | - |
dc.identifier.scopusid | 2-s2.0-65549169136 | - |
dc.author.google | Nam S. | - |
dc.author.google | Min K. | - |
dc.author.google | Hwang H. | - |
dc.author.google | Lee H.-O. | - |
dc.author.google | Lee J.H. | - |
dc.author.google | Yoon J. | - |
dc.author.google | Lee H. | - |
dc.author.google | Park S. | - |
dc.author.google | Lee J. | - |
dc.contributor.scopusid | 박성수(9275920900;41262153700) | - |
dc.date.modifydate | 20230627101106 | - |