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N-Methyl-d-Aspartate Receptor-Mediated Chemotaxis and Ca2+ Signaling in Tetrahymena pyriformis

Title
N-Methyl-d-Aspartate Receptor-Mediated Chemotaxis and Ca2+ Signaling in Tetrahymena pyriformis
Authors
Nam S.-W.Kim S.-T.Lee K.-M.Kim S.H.Kou S.Lim J.Hwang H.Joo M.K.Jeong B.Yoo S.H.Park S.
Ewha Authors
정병문박성수남성원김상태
SCOPUS Author ID
정병문scopus
Issue Date
2009
Journal Title
Protist
ISSN
1434-4610JCR Link
Citation
vol. 160, no. 2, pp. 331 - 342
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Although the ciliate Tetrahymena is a good model for the study of chemotaxis, its profound motility makes it difficult to monitor intracellular calcium (Ca2+) changes induced by chemotactic stimuli. In this study, we report a microfluidic-based chemotaxis system generating directional chemotactic gradients under highly viscous conditions, suppressing T. pyriformis motility, and allowing for the stable confocal imaging of changes in intracellular Ca2+ in the ciliate. Once the viscous condition was achieved, directional chemical gradients were formed inside the center chamber via the release of N-methyl-d-aspartate (NMDA), a known chemoattractant, from the surrounding chemical reservoirs into the center chamber. As a result, intracellular Ca2+ in the ciliate increased up to three-fold, and its distribution was skewed in the direction of NMDA stimulation. However, the Ca2+ in ciliates pretreated with phospholipase C (PLC) or phosphatidylinositol-3-kinase (PI3K) blockers did not increase even after stimulation. Additionally, the PI3K blocker induced the secretion of granules, the size of which was dependent on the concentration of the blocker. Collectively, the results indicate that both PLC and PI3K perform pivotal roles in controlling the levels of intracellular Ca2+ in T. pyriformis during chemotaxis. © 2008 Elsevier GmbH. All rights reserved.
DOI
10.1016/j.protis.2008.10.005
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자연과학대학 > 화학·나노과학전공 > Journal papers
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