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Halostella salina gen. nov., sp nov., an extremely halophilic archaeon isolated from solar salt

Title
Halostella salina gen. nov., sp nov., an extremely halophilic archaeon isolated from solar salt
Authors
Song, Hye SeonCha, In-TaeRhee, Jin-KyuYim, Kyung JuneKim, Ah YoonChoi, Jong-SoonBaek, Su JeongSeo, Myung-JiPark, Soo-JeNam, Young-DoRoh, Seong Woon
Ewha Authors
이진규
SCOPUS Author ID
이진규scopus
Issue Date
2016
Journal Title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
ISSN
1466-5026JCR Link1466-5034JCR Link
Citation
vol. 66, pp. 2740 - 2746
Publisher
MICROBIOLOGY SOC
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
A novel halophilic archaeon designated strain CBA1114(T) was isolated from solar salt in the Republic of Korea. Strain CBA1114(T), cells of which were coccoid and Gram-stain-negative, grew in the presence of 15-30% (w/v) NaCl (optimum, 20%) and at 20-50 degrees C (optimum, 40 degrees C) and pH 7.0-9.0 (optimum, pH 8.0). Strain CBA1114(T) required Mg2+ for growth. Strain CBA1114(T) had three 16S rRNA genes, rrnA, rrnB and rrnC; levels of similarity between the sequences were 99.7-99.9%. The 16S rRNA gene sequence of strain CBA1114(T) showed 91.7% similarity to that of Haloterrigena thermotolerans PR5(T). In multilocus sequence analysis (MLSA), five housekeeping genes, atpB, EF-2, radA, rpoB' and secY, were found to be closely related to those of the members of the genera Halorientalis(89.7 % similarity of the atpB gene sequence), Halomicroarcula(91.9%, EF-2), Haloterrigena(85.4 %, radA), Natronoarchaeum (89.2%, rpoB') and Natrinema(75.7%, secY). A phylogenetic tree generated from the results of MLSA of the five housekeeping genes showed that strain CBA1114(T) was closely related to species of the genus Halorientalis in the family Halobacteriaceae. The major polar lipids were identified as phosphatidylglycerol, phosphatidylglycerolphosphate methyl ester and unidentified lipids. The G+C content of the genomic DNA of strain CBA1114(T) was 68.1 mol%. According to the results of phylogenetic, phenotypic and chemotaxonomic analyses, we designate strain CBA1114(T) (=JCM 30111(T)=KCTC 4206(T)) as the type strain of Halostella salina gen. nov., sp. nov., a novel species of a new genus within the family Halobacteriaceae.
DOI
10.1099/ijsem.0.001118
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엘텍공과대학 > 식품공학전공 > Journal papers
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