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Troglitazone enhances the apoptotic response of DLD-1 colon cancer cells to photodynamic therapy

Title
Troglitazone enhances the apoptotic response of DLD-1 colon cancer cells to photodynamic therapy
Authors
Park H.Ko S.-H.Lee J.M.Park J.H.Choi Y.-H.
Ewha Authors
최윤희박현주
SCOPUS Author ID
최윤희scopus; 박현주scopus
Issue Date
2016
Journal Title
Yonsei Medical Journal
ISSN
0513-5796JCR Link
Citation
vol. 57, no. 6, pp. 1494 - 1499
Keywords
ApoptosisPDTTroglitazone
Publisher
Yonsei University College of Medicine
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
Purpose: The aim of this study was to investigate whether the peroxisomal proliferator-activated receptor gamma (PPARγ) ligand troglitazone in combination with photodynamic therapy (PDT) enhances the apoptotic response of DLD-1 colon cancer cells. Materials and Methods: The effects of troglitazone, PDT, and troglitazone in combination with PDT on cell viability and apoptosis were assessed in DLD-1 cells. Cell viability and proliferation were evaluated using the tetrazolium-based MTT assay, and apoptosis was evaluated via cell staining with propidium iodide (PI) and annexin V-FITC. The levels of pro-caspase-3 were measured via Western blot analyses. Results: Treatment of troglitazone and PDT induced the growth retardation and cell death of DLD-1 cells in a dose-dependent manner, respectively. The combination treatment significantly suppressed cell growth and increased the apoptotic response of DLD-1 and resulted in apoptosis rather than necrosis, as shown by PI/annexin V staining and degradation of procaspase-3. Conclusion: These results document the anti-proliferative and apoptotic activities of PDT in combination with the PPARγ ligand troglitazone and provide a strong rationale for testing the therapeutic potential of combination treatment in colon cancer. © Yonsei University College of Medicine 2016.
DOI
10.3349/ymj.2016.57.6.1494
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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