Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 신정아 | * |
dc.date.accessioned | 2016-09-10T01:09:25Z | - |
dc.date.available | 2016-09-10T01:09:25Z | - |
dc.date.issued | 2015 | * |
dc.identifier.issn | 0146-0404 | * |
dc.identifier.other | OAK-16303 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/232191 | - |
dc.description.abstract | PURPOSE. Previous studies discovered cone phototransduction shutoff occurs normally for Arr1-/- and Arr4-/-; however, it is defective when both visual arrestins are simultaneously not expressed (Arr1-/-Arr4-/-). We investigated the roles of visual arrestins in an all-cone retina (Nrl-/-) since each arrestin has differential effects on visual function, including ARR1 for normal light adaptation, and ARR4 for normal contrast sensitivity and visual acuity. METHODS. We examined Nrl-/-, Nrl-/-Arr1-/-, Nrl-/-Arr4-/-, and Nrl-/-Arr1-/-Arr4-/- mice with photopic electroretinography (ERG) to assess light adaptation and retinal responses, immunoblot and immunohistochemical localization analysis to measure retinal expression levels of M- and S-opsin, and optokinetic tracking (OKT) to measure the visual acuity and contrast sensitivity. RESULTS. Study results indicated that Nrl-/- and Nrl-/-Arr4-/- mice light adapted normally, while Nrl-/-Arr1-/- and Nrl-/-Arr1-/-Arr4-/- mice did not. Photopic ERG a-wave, bwave, and flicker amplitudes followed a general pattern in which Nrl-/-Arr4-/- amplitudes were higher than the amplitudes of Nrl-/-, while the amplitudes of Nrl-/-Arr1-/- and Nrl-/-Arr1-/-Arr4-/- were lower. All three visual arrestin knockouts had faster implicit times than Nrl-/- mice. M-opsin expression is lower when ARR1 is not expressed, while Sopsin expression is lower when ARR4 is not expressed. Although M-opsin expression is mislocalized throughout the photoreceptor cells, S-opsin is confined to the outer segments in all genotypes. Contrast sensitivity is decreased when ARR4 is not expressed, while visual acuity was normal except in Nrl-/-Arr1-/-Arr4-/-. CONCLUSIONS. Based on the opposite visual phenotypes in an all-cone retina in the Nrl-/-Arr1-/- and Nrl-/-Arr4-/- mice,we conclude that ARR1 and ARR4 perform unique modulatory roles in cone photoreceptors. © 2015 The Association for Research in Vision and Ophthalmology, Inc. | * |
dc.language | English | * |
dc.publisher | Association for Research in Vision and Ophthalmology Inc. | * |
dc.subject | Cone function | * |
dc.subject | Cone opsins | * |
dc.subject | NRL knockout | * |
dc.subject | Visual arrestins | * |
dc.title | Arrestin 1 and cone arrestin 4 have unique roles in visual function in an all-cone mouse retina | * |
dc.type | Article | * |
dc.relation.issue | 13 | * |
dc.relation.volume | 56 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 7618 | * |
dc.relation.lastpage | 7628 | * |
dc.relation.journaltitle | Investigative Ophthalmology and Visual Science | * |
dc.identifier.doi | 10.1167/iovs.15-17832 | * |
dc.identifier.wosid | WOS:000368243800002 | * |
dc.identifier.scopusid | 2-s2.0-84982685884 | * |
dc.author.google | Deming J.D. | * |
dc.author.google | Pak J.S. | * |
dc.author.google | Shin J.-A. | * |
dc.author.google | Brown B.M. | * |
dc.author.google | Kim M.K. | * |
dc.author.google | Aung M.H. | * |
dc.author.google | Lee E.-J. | * |
dc.author.google | Pardue M.T. | * |
dc.author.google | Craft C.M. | * |
dc.contributor.scopusid | 신정아(56559179800) | * |
dc.date.modifydate | 20240222145329 | * |