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Isolation of a ventricle-specific promoter for the zebrafish ventricular myosin heavy chain (vmhc) gene and its regulation by GATA factors during embryonic heart development

Title
Isolation of a ventricle-specific promoter for the zebrafish ventricular myosin heavy chain (vmhc) gene and its regulation by GATA factors during embryonic heart development
Authors
Park J.-S.Kim H.-S.Kim J.-D.Seo J.Chung K.-S.Lee H.-S.Huh T.-L.Jo I.Kim Y.-O.
Ewha Authors
조인호
SCOPUS Author ID
조인호scopus
Issue Date
2009
Journal Title
Developmental Dynamics
ISSN
1058-8388JCR Link
Citation
vol. 238, no. 6, pp. 1574 - 1581
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
We investigated chamber-specific gene expression by isolating a 2.2-kb polymerase chain reaction product containing the 5 ′-flanking region of the zebrafish ventricular myosin heavy-chain gene (vmhc). Promoter analysis revealed that the fragment, consisting of nucleotides from -301 to +26, is sufficient for vmhc promoter activity. Among several putative cis-acting elements in the region, a GATA-binding site was identified to be crucial for the activity of the promoter, as evidenced by the complete abolishment of promoter activity by a single nucleotide substitution of GATA-binding site (-287, C→T). Knockdown of GATA-binding proteins 4 and 6 (GATA4 and -6) by their antisense morpholino oligonucleotides resulted in reduced green fluorescent protein (GFP) reporter gene and endogenous vmhc expression. These findings suggest that GATA4 and -6 play a key role in the regulation of vmhc expression in the ventricle. In addition, the vmhc promoter and the transgenic zebrafish (vmhc:gfp) are useful tools to study the formation and function of the ventricle. © 2009 Wiley-Liss, Inc.
DOI
10.1002/dvdy.21964
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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