Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 조인호 | * |
dc.date.accessioned | 2016-08-29T11:08:42Z | - |
dc.date.available | 2016-08-29T11:08:42Z | - |
dc.date.issued | 2009 | * |
dc.identifier.issn | 1058-8388 | * |
dc.identifier.other | OAK-5670 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/231950 | - |
dc.description.abstract | We investigated chamber-specific gene expression by isolating a 2.2-kb polymerase chain reaction product containing the 5 ′-flanking region of the zebrafish ventricular myosin heavy-chain gene (vmhc). Promoter analysis revealed that the fragment, consisting of nucleotides from -301 to +26, is sufficient for vmhc promoter activity. Among several putative cis-acting elements in the region, a GATA-binding site was identified to be crucial for the activity of the promoter, as evidenced by the complete abolishment of promoter activity by a single nucleotide substitution of GATA-binding site (-287, C→T). Knockdown of GATA-binding proteins 4 and 6 (GATA4 and -6) by their antisense morpholino oligonucleotides resulted in reduced green fluorescent protein (GFP) reporter gene and endogenous vmhc expression. These findings suggest that GATA4 and -6 play a key role in the regulation of vmhc expression in the ventricle. In addition, the vmhc promoter and the transgenic zebrafish (vmhc:gfp) are useful tools to study the formation and function of the ventricle. © 2009 Wiley-Liss, Inc. | * |
dc.language | English | * |
dc.title | Isolation of a ventricle-specific promoter for the zebrafish ventricular myosin heavy chain (vmhc) gene and its regulation by GATA factors during embryonic heart development | * |
dc.type | Article | * |
dc.relation.issue | 6 | * |
dc.relation.volume | 238 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 1574 | * |
dc.relation.lastpage | 1581 | * |
dc.relation.journaltitle | Developmental Dynamics | * |
dc.identifier.doi | 10.1002/dvdy.21964 | * |
dc.identifier.wosid | WOS:000266678100030 | * |
dc.identifier.scopusid | 2-s2.0-66349097757 | * |
dc.author.google | Park J.-S. | * |
dc.author.google | Kim H.-S. | * |
dc.author.google | Kim J.-D. | * |
dc.author.google | Seo J. | * |
dc.author.google | Chung K.-S. | * |
dc.author.google | Lee H.-S. | * |
dc.author.google | Huh T.-L. | * |
dc.author.google | Jo I. | * |
dc.author.google | Kim Y.-O. | * |
dc.contributor.scopusid | 조인호(26643129000;56663841900) | * |
dc.date.modifydate | 20240123112949 | * |