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Engineering of plant-specific phenylpropanoids biosynthesis in Streptomyces venezuelae

Title
Engineering of plant-specific phenylpropanoids biosynthesis in Streptomyces venezuelae
Authors
Park S.R.Yoon J.A.Paik J.H.Park J.W.Jung W.S.Ban Y.-H.Kim E.J.Yoo Y.J.Han A.R.Yoon Y.J.
Ewha Authors
윤여준
SCOPUS Author ID
윤여준scopus
Issue Date
2009
Journal Title
Journal of Biotechnology
ISSN
0168-1656JCR Link
Citation
vol. 141, no. 41337, pp. 181 - 188
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Phenylpropanoids, including flavonoids and stilbenes, are plant secondary metabolites with potential pharmacological and nutraceutical properties. To expand the applicability of Streptomyces venezuelae as a heterologous host to plant polyketide production, flavonoid and stilbene biosynthetic genes were expressed in an engineered strain of S. venezuelae DHS2001 bearing a deletion of native pikromycin polyketide synthase gene. A plasmid expressing the 4-coumarate/cinnamate:coenzyme A ligase from Streptomyces coelicolor (ScCCL) and the chalcone synthase from Arabidopsis thaliana (atCHS) under the control of a single ermE* promoter was constructed and introduced into S. venezuelae DHS2001. The resulting strain produced racemic naringenin and pinocembrin from 4-coumaric acid and cinnamic acid, respectively. Placement of an additional ermE* promoter upstream of the codon-optimized atCHS (atCHS op) gene significantly increased the yield of both flavanones. Expression of codon-optimized chalcone isomerase gene from Medicago sativa, together with ScCCL and atCHS op genes led to production of (2S)-flavanones, but the yield was reduced. On the other hand, a recombinant strain harboring the ScCCL and codon-optimized stilbene synthase gene from Arachis hypogaea generated stilbenes such as resveratrol and pinosylvin. This is the first report on the heterologous expression of plant phenylpropanoid biosynthetic pathways in Streptomyces genus. © 2009 Elsevier B.V. All rights reserved.
DOI
10.1016/j.jbiotec.2009.03.013
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자연과학대학 > 화학·나노과학전공 > Journal papers
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