View : 9 Download: 3

Proton pump inhibitors exert anti-allergic effects by reducing TCTP secretion

Title
Proton pump inhibitors exert anti-allergic effects by reducing TCTP secretion
Authors
Choi S.Min H.J.Kim M.Hwang E.S.Lee K.
Ewha Authors
이경림황은숙
SCOPUS Author ID
이경림scopus; 황은숙scopus
Issue Date
2009
Journal Title
PLoS ONE
ISSN
1932-6203JCR Link
Citation
vol. 4, no. 6
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Background: Extracellular translationally controlled tumor protein (TCTP) is known to play a role in human allergic responses. TCTP has been identified outside of macrophages, in activated mononuclear cells, and in biological fluids from allergic patients. Even TCTP devoid of signal sequences, is secreted to extracellular environment by an yet undefined mechanism. This study is aimed at understanding the mechanism of TCTP release and its regulation. A secondary goal is to see if inhibitors of TCTP release can serve as potential anti-allergic asthmatic drugs. Methodology/Principal Findings: Using Western blotting assay in HEK293 and U937 cells, we found that TCTP secretion is reduced by omeprazole and pantoprazole, both of which are proton pump inhibitors. We then transfected HEK293 cells with proton pump expression vectors to search for the effects of exogeneously overexpressed H+/K+-ATPase on the TCTP secretion. Based on these in vitro data we checked the in vivo effects of pantoprazole in a murine model of ovalbumin-induced allergy. Omeprazole and pantoprazole reduced TCTP secretion from HEK293 and U937 cells in a concentration-dependent fashion and the secretion of TCTP from HEK293 cells increased when they over-expressed H+/K+-ATPase. In a murine model of ovalbumin-induced allergy, pretreatment with pantoprazole reduced infiltration of inflammatory cells, increased goblet cells, and increased TCTP secretion induced by OVA challenge. Conclusion: Since Omeprazole and pantoprazole decrease the secretion of TCTP which is associated with the development of allergic reaction, they may have the potential to serve as anti-allergic (asthmatic) drugs. © 2009 Choi et al.
DOI
10.1371/journal.pone.0005732
Appears in Collections:
약학대학 > 약학과 > Journal papers
Files in This Item:
Proton pump inhibitors exert anti-allergic effects by reducing TCTP secretion.PDF(909.09 kB)Download
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE