Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 황금숙 | * |
dc.date.accessioned | 2016-08-29T12:08:48Z | - |
dc.date.available | 2016-08-29T12:08:48Z | - |
dc.date.issued | 2016 | * |
dc.identifier.issn | 1949-2553 | * |
dc.identifier.other | OAK-19184 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/231832 | - |
dc.description.abstract | Abnormal tumor cell metabolism is a consequence of alterations in signaling pathways that provide critical selective advantage to cancer cells. However, a systematic characterization of the metabolic and signaling pathways altered in cancer stem-like cells (CSCs) is currently lacking. Using nuclear magnetic resonance and mass spectrometry, we profiled the whole-cell metabolites of a pair of parental (P-231) and stem-like cancer cells (S-231), and then integrated with whole transcriptome profiles. We identified elevated NAAD(+) in S-231 along with a coordinated increased expression of genes in Wnt/calcium signaling pathway, reflecting the correlation between metabolic reprogramming and altered signaling pathways. The expression of CD38 and ALP, upstream NAAD(+) regulatory enzymes, was oppositely regulated between P-and S-231; high CD38 strongly correlated with NAADP in P-231 while high ALP with NAAD(+) levels in S-231. Antagonizing Wnt activity by dnTCF4 transfection reversed the levels of NAAD(+) and ALP expression in S-231. Of note, elevated NAAD(+) caused a decrease of cytosolic Ca2+ levels preventing calcium-induced apoptosis in nutrient-deprived conditions. Reprograming of NAD(+) metabolic pathway instigated by Wnt signaling prevented cytosolic Ca2+ overload thereby inhibiting calcium-induced apoptosis in S-231. These results suggest that "oncometabolites" resulting from cross talk between the deranged core cancer signaling pathway and metabolic network provide a selective advantage to CSCs. | * |
dc.language | English | * |
dc.publisher | IMPACT JOURNALS LLC | * |
dc.subject | calcium signaling | * |
dc.subject | cancer stem cells | * |
dc.subject | integrated analysis | * |
dc.subject | metabolic reprogramming | * |
dc.subject | Wnt signaling | * |
dc.title | Integrated omics-analysis reveals Wnt-mediated NAD(+) metabolic reprogramming in cancer stem-like cells | * |
dc.type | Article | * |
dc.relation.issue | 30 | * |
dc.relation.volume | 7 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 48562 | * |
dc.relation.lastpage | 48576 | * |
dc.relation.journaltitle | ONCOTARGET | * |
dc.identifier.doi | 10.18632/oncotarget.10432 | * |
dc.identifier.wosid | WOS:000385413000134 | * |
dc.identifier.scopusid | 2-s2.0-84982832249 | * |
dc.author.google | Lee, Jueun | * |
dc.author.google | Kee, Hyun Jung | * |
dc.author.google | Min, Soonki | * |
dc.author.google | Park, Ki Cheong | * |
dc.author.google | Park, Sunho | * |
dc.author.google | Hwang, Tae Hyun | * |
dc.author.google | Ryu, Do Hyun | * |
dc.author.google | Hwang, Geum-Sook | * |
dc.author.google | Cheong, Jae-Ho | * |
dc.contributor.scopusid | 황금숙(7202676099) | * |
dc.date.modifydate | 20240222154747 | * |