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Early diagnostic method of avian influenza virus subtype using ultra real-time PCR

Title
Early diagnostic method of avian influenza virus subtype using ultra real-time PCR
Authors
Kim S.-T.Kim Y.K.Kim J.S.
Ewha Authors
김상태
Issue Date
2011
Journal Title
Korean Journal of Microbiology
ISSN
0440-2413JCR Link
Citation
vol. 47, no. 1, pp. 30 - 37
Indexed
SCOPUS; KCI scopus
Abstract
This ultra real-time PCR (UPCR) based diagnosis system for avian influenza A virus (AIV) subtype was designed. The target primer in this study was derived from H5N1 subtype-specific 133 bp partial gene of hemagglutinin (HA), and was synthesized by using PCR-based gene synthesis on the ground of safety. UPCR was operated by Mini-Opticon Q-PCR Quantitative Thermal Cycler using aptamer-based molecular beacon, total 10 μl of reaction mixture with extraordinarily short time in each steps in PCR. The detection including UPCR and analysis of melting temperature was totally operated within 15 min. The AIV-specific 133 bp PCR product was correctly amplified until 5 molecules of HA gene as minimum of templates. This kind of PCR was drafted as UPCR in this study and it could be used to detect not only AIV subtype, but also other pathogens using UPCR-based diagnosis. © 2011, The Microbiological Society of Korea.
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자연과학대학 > 화학·나노과학전공 > Journal papers
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